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Product Info Summary
| SKU: | A10947 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LSM8 Antibody Picoband®
SKU/Catalog Number
A10947
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LSM8 Antibody Picoband® catalog # A10947. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LSM8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10947)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LSM8 recombinant protein (Position: M1-H96).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10947 is reactive to LSM8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
10.403kDa
Background of LSM8
U6 snRNA-associated Sm-like protein LSm8 is a protein that in humans is encoded by the LSM8 gene. This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10947 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HELA whole cell, human HL-60 whole cell, human HEPG2 whole cell, human U937 whole cell, human Jurkat whole cell, human Raji whole cell, human THP-1 whole cell, human K562 whole cell, rat testis tissue, rat lung tissue, rat liver tissue, rat pancreas tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, mouse HEPA1-6 whole cell
IHC: human skin cancer tissue, mouse testis tissue, rat testis tissue, human ovary cancer tissue, human appendicitis tissue, human renal carcinoma tissue, human gastric cancer tissue, human melanoma tissue, human bladder cancer tissue, human tonsil tissue
ICC/IF: A431 cell
FCM: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LSM8 using anti-LSM8 antibody (A10947).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human HEPG2 whole cell lysates,
Lane 4: human U937 whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human Raji whole cell lysates,
Lane 7: human THP-1 whole cell lysates,
Lane 8: human K562 whole cell lysates,
Lane 9: rat testis tissue lysates,
Lane 10: rat lung tissue lysates,
Lane 11: rat liver tissue lysates,
Lane 12: rat pancreas tissue lysates,
Lane 13: mouse testis tissue lysates,
Lane 14: mouse lung tissue lysates,
Lane 15: mouse liver tissue lysates,
Lane 16: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM8 antigen affinity purified polyclonal antibody (Catalog # A10947) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM8 at approximately 16KD. The expected band size for LSM8 is at 11KD.
Click image to see more details
Figure 10. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 11. IHC analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 12. IF analysis of LSM8 using anti-LSM8 antibody (A10947).
LSM8 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-LSM8 Antibody (A10947) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 13. Flow Cytometry analysis of A431 cells using anti-LSM8 antibody (A10947).
Overlay histogram showing A431 cells stained with A10947 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LSM8 Antibody (A10947, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For LSM8 (Source: Uniprot.org, NCBI)
Gene Name
LSM8
Full Name
U6 snRNA-associated Sm-like protein LSm8
Weight
10.403kDa
Superfamily
snRNP Sm proteins family
Alternative Names
Interleukin-17B; IL-17B; Cytokine CX1; Cytokine-like protein ZCYTO7; Neuronal interleukin-17-related factor; Il17b; Nirf, Zcyto7 LSM8 NAA38 LSM8 homolog, U6 small nuclear RNA associated LSM8 homolog, U6 small nuclear RNA associated|LSM8 U6 small nuclear RNA associated|MAK31-like protein|N-alpha-acetyltransferase 38, NatC auxiliary subunit|U6 snRNA-associated Sm-like protein LSm8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LSM8, check out the LSM8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LSM8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LSM8 Antibody Picoband® (A10947)
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