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Product Info Summary
| SKU: | A02050 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Lsk MATK Monoclonal Antibody
SKU/Catalog Number
A02050
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Lsk MATK Monoclonal Antibody catalog # A02050. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lsk MATK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02050)
Host
Mouse
Contents
Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
9D7
Isotype
IgG
Immunogen
Purified recombinant fragment of human Lsk expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02050 is reactive to MATK in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
56469 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02050 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
FC, 1:200-1:400
Validation Images & Assay Conditions
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Western Blot (WB) analysis using Lsk Monoclonal antibody against K562 cell lysate (1).
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Flow cytometric (FCM) analysis of K562 cells using Lsk Monoclonal antibody (green) and negative control (purple).
Protein Target Info & Infographic
Gene/Protein Information For MATK (Source: Uniprot.org, NCBI)
Gene Name
MATK
Full Name
Megakaryocyte-associated tyrosine-protein kinase
Weight
56469 MW
Superfamily
protein kinase superfamily
Alternative Names
Megakaryocyte-associated tyrosine-protein kinase;2.7.10.2;CSK homologous kinase;CHK;Hematopoietic consensus tyrosine-lacking kinase;Protein kinase HYL;Tyrosine-protein kinase CTK;MATK;CTK, HYL; MATK CHK, CTK, HHYLTK, HYL, HYLTK, Lsk megakaryocyte-associated tyrosine kinase megakaryocyte-associated tyrosine-protein kinase|CSK homologous kinase|Csk-homologous kinase|Csk-type protein tyrosine kinase|HYL tyrosine kinase|hematopoietic consensus tyrosine-lacking kinase|hydroxyaryl-protein kinase|leukocyte carboxyl-terminal src kinase related|protein kinase HYL|tyrosine kinase MATK|tyrosine-protein kinase CTK|tyrosylprotein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MATK, check out the MATK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MATK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lsk MATK Monoclonal Antibody (A02050)
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No publications found for A02050
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Customer Q&As
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5 Customer Q&As for Anti-Lsk MATK Monoclonal Antibody
Question
We are currently using anti-Lsk Monoclonal antibody A02050 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
The anti-Lsk Monoclonal antibody (A02050) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-04
Question
I see that the anti-Lsk Monoclonal antibody A02050 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-17
Question
Would A02050 anti-Lsk Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
As indicated on the product datasheet, A02050 anti-Lsk Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-09
Question
Would anti-Lsk Monoclonal antibody A02050 work on monkey Flow Cytometry with testis?
C. Singh
Verified customer
Asked: 2015-08-20
Answer
Our lab technicians have not validated anti-Lsk Monoclonal antibody A02050 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Lsk Monoclonal antibody A02050 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey testis in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-20
Question
I was wanting to use your anti-Lsk Monoclonal antibody for Flow Cytometry for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
B. Krishna
Verified customer
Asked: 2014-10-20
Answer
It shows on the product datasheet, A02050 anti-Lsk Monoclonal antibody has been tested for Flow Cytometry, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-10-20
