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Product Info Summary
| SKU: | A04854 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-LRRFIP1 Monoclonal Antibody
SKU/Catalog Number
A04854
Size
100μl
Form
Liquid
Description
Boster Bio Anti-LRRFIP1 Monoclonal Antibody catalog # A04854. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LRRFIP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04854)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
7C1
Isotype
IgG
Immunogen
Purified recombinant human LRRFIP1 protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04854 is reactive to LRRFIP1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
89253 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04854 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using LRRFIP1 Monoclonal antibody against Raji, C2C12, MCF7, Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For LRRFIP1 (Source: Uniprot.org, NCBI)
Gene Name
LRRFIP1
Full Name
Leucine-rich repeat flightless-interacting protein 1
Weight
89253 MW
Superfamily
LRRFIP family
Alternative Names
Leucine-rich repeat flightless-interacting protein 1;LRR FLII-interacting protein 1;GC-binding factor 2;TAR RNA-interacting protein;LRRFIP1;GCF2, TRIP; LRRFIP1 FLAP-1, FLAP1, FLIIAP1, GCF-2, GCF2, HUFI-1, TRIP LRR binding FLII interacting protein 1 leucine-rich repeat flightless-interacting protein 1|GC-binding factor 2|LRR FLII-interacting protein 1|NEDD8-conjugating enzyme|TAR RNA-interacting protein|leucine rich repeat (in FLII) interacting protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRFIP1, check out the LRRFIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRFIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRFIP1 Monoclonal Antibody (A04854)
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No publications found for A04854
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-LRRFIP1 Monoclonal Antibody
Question
I am interested in to test anti-LRRFIP1 Monoclonal antibody A04854 on human melanoma for research purposes, then I may be interested in using anti-LRRFIP1 Monoclonal antibody A04854 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
The products we sell, including anti-LRRFIP1 Monoclonal antibody A04854, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-05
Question
Would anti-LRRFIP1 Monoclonal antibody A04854 work on pig WB with liver?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Our lab technicians have not validated anti-LRRFIP1 Monoclonal antibody A04854 on pig. You can run a BLAST between pig and the immunogen sequence of anti-LRRFIP1 Monoclonal antibody A04854 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-LRRFIP1 Monoclonal antibody A04854. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-21
Question
Is a blocking peptide available for product anti-LRRFIP1 Monoclonal antibody (A04854)?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
We do provide the blocking peptide for product anti-LRRFIP1 Monoclonal antibody (A04854). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-15
Question
Is there a BSA free version of anti-LRRFIP1 Monoclonal antibody A04854 available?
C. Roberts
Verified customer
Asked: 2019-02-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-LRRFIP1 Monoclonal antibody A04854 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-28
Question
Here is the WB image, lot number and protocol we used for melanoma using anti-LRRFIP1 Monoclonal antibody A04854. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-28
Question
Is this A04854 anti-LRRFIP1 Monoclonal antibody reactive to the isotypes of LRRFIP1?
K. Evans
Verified customer
Asked: 2018-05-04
Answer
The immunogen of A04854 anti-LRRFIP1 Monoclonal antibody is Purified recombinant human LRRFIP1 protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-04
Question
Will A04854 anti-LRRFIP1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
It shows on the product datasheet, A04854 anti-LRRFIP1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-30
Question
I see that the anti-LRRFIP1 Monoclonal antibody A04854 works with WB, what is the protocol used to produce the result images on the product page?
V. Li
Verified customer
Asked: 2017-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-28
Question
We are currently using anti-LRRFIP1 Monoclonal antibody A04854 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The anti-LRRFIP1 Monoclonal antibody (A04854) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-19
Question
I have a question about product A04854, anti-LRRFIP1 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Williams
Verified customer
Asked: 2016-07-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04854 anti-LRRFIP1 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-07-01
Question
Will anti-LRRFIP1 Monoclonal antibody A04854 work for WB with melanoma?
J. Edwards
Verified customer
Asked: 2016-05-27
Answer
According to the expression profile of melanoma, LRRFIP1 is highly expressed in melanoma. So, it is likely that anti-LRRFIP1 Monoclonal antibody A04854 will work for WB with melanoma.
Boster Scientific Support
Answered: 2016-05-27
Question
I was wanting to use your anti-LRRFIP1 Monoclonal antibody for WB for human melanoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human melanoma identification?
J. Mitchell
Verified customer
Asked: 2014-01-28
Answer
As indicated on the product datasheet, A04854 anti-LRRFIP1 Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-28
