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Product Info Summary
| SKU: | M00829 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal Antibody
SKU/Catalog Number
M00829
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal Antibody catalog # M00829. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00829)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BDC-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LRP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00829 is reactive to LRP1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
20 kDa
Calculated molecular weight
504606 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00829 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:20000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:30
FC 1:50
Positive Control
IHC: human liver carcinoma tissue, Human pituitary tumor tissue, Human prostate cancer tissue, Human breast cancer tissue
FCM: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of LRP1 expression in A549 cell lysate.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human liver carcinoma, using LRP1 Antibody.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:300 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Flow cytometric analysis of LRP1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Protein Target Info & Infographic
Gene/Protein Information For LRP1 (Source: Uniprot.org, NCBI)
Gene Name
LRP1
Full Name
Prolow-density lipoprotein receptor-related protein 1
Weight
504606 MW
Superfamily
LDLR family
Alternative Names
Prolow-density lipoprotein receptor-related protein 1;LRP-1;Alpha-2-macroglobulin receptor;A2MR;Apolipoprotein E receptor;APOER;CD91;Low-density lipoprotein receptor-related protein 1 85 kDa subunit;LRP-85;Low-density lipoprotein receptor-related protein 1 515 kDa subunit;LRP-515;Low-density lipoprotein receptor-related protein 1 intracellular domain;LRPICD;LRP1;A2MR, APR; LRP1 A2MR, APOER, APR, CD91, IGFBP-3R, IGFBP3R, IGFBP3R1, KPA, LRPA, TGFBR5, LRP1 LDL receptor related protein 1 prolow-density lipoprotein receptor-related protein 1|TbetaR-V/LRP-1/IGFBP-3 receptor|alpha-2-macroglobulin receptor|apolipoprotein E receptor|low density lipoprotein receptor-related protein 1|type V tgf-beta receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRP1, check out the LRP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal Antibody (M00829)
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No publications found for M00829
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Customer Q&As
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5 Customer Q&As for Anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal Antibody
Question
We are currently using anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody M00829 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody (M00829) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-29
Question
Is a blocking peptide available for product anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody (M00829)?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do provide the blocking peptide for product anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody (M00829). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody M00829. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-06
Question
Is this M00829 anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody reactive to the isotypes of LRP1?
Verified Customer
Verified customer
Asked: 2017-11-29
Answer
The immunogen of M00829 anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody is A synthesized peptide derived from human LRP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-29
Question
Does anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody M00829 work on primate IHC with plasma?
J. Anderson
Verified customer
Asked: 2013-01-24
Answer
Our lab technicians have not validated anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody M00829 on primate. You can run a BLAST between primate and the immunogen sequence of anti-LRP1/Lrp 1 Cluster Ii Rabbit Monoclonal antibody M00829 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate plasma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-24
