Product Info Summary
SKU: | PA1833 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-LOX 1/OLR1 Antibody Picoband®
View all LOX-1/OLR1 Antibodies
SKU/Catalog Number
PA1833
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LOX 1/OLR1 Antibody catalog # PA1833. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LOX 1/OLR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1833)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human LOX 1, different from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1833 is reactive to OLR1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48-52 kDa
Calculated molecular weight
30959 MW
Background of LOX-1/OLR1
OLR1 (oxidized low density lipoprotein (lectin-like) receptor 1) also called CLEC8A, LOX-1, SCARE1, is a receptor protein which belongs to the C-type lectin superfamily. The OLR1 gene encodes a cell-surface endocytosis receptor for oxidized low density lipoprotein (OxLDL). The OLR1 gene is mapped on 12p13.2. Incubation of the cells with LDL had no effect on LOX1 expression, but incubation with OxLDL resulted in a dose-dependent increase in LOX1 mRNA and protein expression; however, very high concentrations of OxLDL caused a decrease in OxLDL expression, perhaps indicating toxic effects on endothelial cells. LOX1 was also expressed in macrophages, but not in vascular smooth muscle cells. The findings suggested a role for LOX1 in the pathophysiology of atherosclerotic cardiovascular disease. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1833 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human prostatic carcinoma tissue (PCT), human cervical carcinoma tissue (CCAT), human gallbladder carcinoma tissue (GBCT), human intrahepatic cholangiocarcinom paracancerous tissue (ICCP)
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LOX 1/OLR1 using anti-LOX 1/OLR1 antibody (PA1833).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human prostatic carcinoma tissue (PCT) lysates,
Lane 2: human cervical carcinoma tissue (CCAT) lysates,
Lane 3: human gallbladder carcinoma tissue (GBCT) lysates,
Lane 4: human intrahepatic cholangiocarcinom paracancerous tissue (ICCP) lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX 1/OLR1 antigen affinity purified polyclonal antibody (Catalog # PA1833) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX 1/OLR1 at approximately 48-52 kDa. The expected band size for LOX 1/OLR1 is at 31 kDa.
Protein Target Info & Infographic
Gene/Protein Information For OLR1 (Source: Uniprot.org, NCBI)
Gene Name
OLR1
Full Name
Oxidized low-density lipoprotein receptor 1
Weight
30959 MW
Alternative Names
Oxidized low-density lipoprotein receptor 1;Ox-LDL receptor 1;C-type lectin domain family 8 member A;Lectin-like oxidized LDL receptor 1;LOX-1;Lectin-like oxLDL receptor 1;hLOX-1;Lectin-type oxidized LDL receptor 1;Oxidized low-density lipoprotein receptor 1, soluble form;OLR1;CLEC8A, LOX1; OLR1 CLEC8A, LOX1, LOXIN, SCARE1, SLOX1 oxidized low density lipoprotein receptor 1 oxidized low-density lipoprotein receptor 1|C-type lectin domain family 8 member A|hLOX-1|lectin-type oxidized LDL receptor 1|ox LDL receptor 1|oxidized low density lipoprotein (lectin-like) receptor 1|oxidized low-density lipoprotein receptor 1, soluble form|scavenger receptor class E, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OLR1, check out the OLR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OLR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LOX 1/OLR1 Antibody Picoband® (PA1833)
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Customer Q&As
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10 Customer Q&As for Anti-LOX 1/OLR1 Antibody Picoband®
Question
I was wanting to use your anti-LOX 1/OLR1 antibody for WB for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
You can see on the product datasheet, PA1833 anti-LOX 1/OLR1 antibody has been validated for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-14
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-LOX 1/OLR1 antibody PA1833. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-05
Question
Will PA1833 anti-LOX 1/OLR1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
It shows on the product datasheet, PA1833 anti-LOX 1/OLR1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-12
Question
Is a blocking peptide available for product anti-LOX 1/OLR1 antibody (PA1833)?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We do provide the blocking peptide for product anti-LOX 1/OLR1 antibody (PA1833). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-10
Question
I see that the anti-LOX 1/OLR1 antibody PA1833 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-13
Question
What is the shelf life PA1833 (ex. number of days, months, years), and at what point is shelf-life measured (ex. date of: manufacture, ship, receipt, or test)?
Verified customer
Asked: 2019-08-12
Answer
At -20°C for one year. After reconstitution, at 4°C for one month. The Anti-LOX 1/OLR1 Antibody (PA1833) can also be aliquotted and stored frozen at -20°C for a longer time. The shelf life is measured at the date of manufacture.
Boster Scientific Support
Answered: 2019-08-12
Question
What is the recommended storage room temperature of PA1833?
Verified customer
Asked: 2019-08-12
Answer
At -20°C for one year. After reconstitution, at 4°C for one month. The Anti-LOX 1/OLR1 Antibody (PA1833) can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Boster Scientific Support
Answered: 2019-08-12
Question
How is PA1833 packaged (ex.: bottle, vial etc.) and what is the packaging material made of (ex.metal, plastic, glass, etc.)?
Verified customer
Asked: 2019-08-12
Answer
The Anti-LOX 1/OLR1 Antibody (PA1833) is in a plastic vial with a plastic packaging that also contains the datasheet.
Boster Scientific Support
Answered: 2019-08-12
Question
How many components are included in PA1833, and what sizes are they (5 x 1 ul, 1 x 2 mg, etc.)?
Verified customer
Asked: 2019-08-12
Answer
The Anti-LOX 1/OLR1 Antibody (PA1833) contains one vial of antibody, 100μg/vial + datasheet.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-LOX 1/OLR1 antibody PA1833 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
D. Miller
Verified customer
Asked: 2017-05-05
Answer
The anti-LOX 1/OLR1 antibody (PA1833) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-05