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Product Info Summary
| SKU: | A11805-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DDX8 DHX8 Antibody
SKU/Catalog Number
A11805-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DDX8 DHX8 Antibody catalog # A11805-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX8 DHX8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11805-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DHX8. AA range:391-440
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11805-1 is reactive to DHX8 in Human, Mouse
Reconstitution
Calculated molecular weight
139315 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11805-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using DDX8 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For DHX8 (Source: Uniprot.org, NCBI)
Gene Name
DHX8
Full Name
ATP-dependent RNA helicase DHX8
Weight
139315 MW
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DHX8;3.6.4.13;DEAH box protein 8;RNA helicase HRH1;DHX8;DDX8; DHX8 DDX8, Dhr2, HRH1, PRP22, PRPF22 DEAH-box helicase 8 ATP-dependent RNA helicase DHX8|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)|DEAH (Asp-Glu-Ala-His) box polypeptide 8|DEAH box protein 8|RNA helicase HRH1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DHX8, check out the DHX8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DHX8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX8 DHX8 Antibody (A11805-1)
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No publications found for A11805-1
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Customer Q&As
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7 Customer Q&As for Anti-DDX8 DHX8 Antibody
Question
We are currently using anti-DDX8 antibody A11805-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The anti-DDX8 antibody (A11805-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-08
Question
Would A11805-1 anti-DDX8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
As indicated on the product datasheet, A11805-1 anti-DDX8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-08
Question
Is a blocking peptide available for product anti-DDX8 antibody (A11805-1)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
We do provide the blocking peptide for product anti-DDX8 antibody (A11805-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-DDX8 antibody A11805-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-05
Question
We are interested in to test anti-DDX8 antibody A11805-1 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-DDX8 antibody A11805-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The products we sell, including anti-DDX8 antibody A11805-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-06
Question
My question regarding product A11805-1, anti-DDX8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Banerjee
Verified customer
Asked: 2018-01-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11805-1 anti-DDX8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-31
Question
I was wanting to use your anti-DDX8 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
S. Zhao
Verified customer
Asked: 2016-10-04
Answer
It shows on the product datasheet, A11805-1 anti-DDX8 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-04
