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Product Info Summary
| SKU: | A00239-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-LGR5 Antibody Picoband®
View all Lgr5/GPR49 Antibodies
SKU/Catalog Number
A00239-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LGR5 Antibody Picoband® catalog # A00239-2. Tested in WB, IHC, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LGR5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00239-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LGR5 recombinant protein (Position: R26-L907). Human LGR5 shares 87% and 87.1% amino acid (aa) sequence identity with mouse and rat LGR5, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00239-2 is reactive to LGR5 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
100,97,92 kDa
Background of Lgr5/GPR49
Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00239-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry, 2-5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human HepG2 whole cell, human RT4 whole cell, human 293T whole cell
IHC: mouse small intestine tissue, rat small intestine tissue
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LGR5 using anti-LGR5 antibody (A00239-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGR5 antigen affinity purified polyclonal antibody (Catalog # A00239-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LGR5 at approximately 130 kDa. The expected band size for LGR5 is at 100,97,92 kDa.
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Figure 2. IHC analysis of LGR5 using anti-LGR5 antibody (A00239-2).
LGR5 was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LGR5 Antibody (A00239-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of LGR5 using anti-LGR5 antibody (A00239-2).
LGR5 was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LGR5 Antibody (A00239-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of 293T cells using anti-LGR5 antibody (A00239-2).
Overlay histogram showing 293T cells stained with A00239-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LGR5 Antibody (A00239-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For LGR5 (Source: Uniprot.org, NCBI)
Gene Name
LGR5
Full Name
Leucine-rich repeat-containing G-protein coupled receptor 5
Weight
100,97,92 kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38 LGR5 FEX, GPR49, GPR67, GRP49, HG38 leucine rich repeat containing G protein-coupled receptor 5 leucine-rich repeat-containing G-protein coupled receptor 5|G-protein coupled receptor 49|G-protein coupled receptor 67|G-protein coupled receptor HG38|orphan G protein-coupled receptor HG38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LGR5, check out the LGR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LGR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LGR5 Antibody Picoband® (A00239-2)
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