Anti-LGR4/Gpr48 Monoclonal Antibody

Product Name

Anti-LGR4/Gpr48 Monoclonal Antibody

View all Lgr4/GPR48 Antibodies

SKU/Catalog Number

M02134

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-LGR4/Gpr48 Monoclonal Antibody (Catalog # M02134). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-LGR4/Gpr48 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02134)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 6G8.B3.G5.C3

Isotype

IgG2b kappa

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M02134 is reactive to LGR4 in Human

Reconstitution

Calculated molecular weight

104475 MW

Background of Lgr4/GPR48

LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02134 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IHC: 5 µg/mL
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

Gene/Protein Information For LGR4 (Source: Uniprot.org, NCBI)

Alternative Names

LGR4, LGR 4, leucine-rich repeat-containing G protein-coupled receptor 4 LGR4 BNMD17, GPR48 leucine rich repeat containing G protein-coupled receptor 4 leucine-rich repeat-containing G-protein coupled receptor 4|G protein-coupled receptor 48

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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