Click image to see more details
Product Info Summary
| SKU: | M06558 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-LEFTY A LEFTY2 Monoclonal Antibody
SKU/Catalog Number
M06558
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-LEFTY A LEFTY2 Monoclonal Antibody (Catalog # M06558). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-LEFTY A LEFTY2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06558)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 7C5G1H6H10 IgG2a kappa
Isotype
IgG2a kappa
Immunogen
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06558 is reactive to LEFTY2 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
40920 MW
Background of LEFTY2
During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06558 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000
WB: 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of LEFTY A expression in unrelated 6X His tagged protein (lane 1) and partially purified recombinant human LEFTY (lane 2). LEFTY A at 30KD was detected using mouse anti-LEFTY A protein A purified monoclonal antibody (Catalog # M06558) at 1:2000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Protein Target Info & Infographic
Gene/Protein Information For LEFTY2 (Source: Uniprot.org, NCBI)
Gene Name
LEFTY2
Full Name
Left-right determination factor 2
Weight
40920 MW
Superfamily
TGF-beta family
Alternative Names
leftyA, protein lefty 2, Left-right determination factor 2, Protein lefty-2, Left-right determination factor A, Protein lefty-A, Transforming growth factor beta-4, TGF-beta-4, Endometrial bleeding-associated factor LEFTY2 EBAF, LEFTA, LEFTYA, TGFB4 left-right determination factor 2 left-right determination factor 2|TGF-beta-4|endometrial bleeding-associated factor|left-right determination factor A|protein lefty-2|protein lefty-A|transforming growth factor beta-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LEFTY2, check out the LEFTY2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LEFTY2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LEFTY A LEFTY2 Monoclonal Antibody (M06558)
Hello CJ!
No publications found for M06558
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-LEFTY A LEFTY2 Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-LEFTY A LEFTY2 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-LEFTY A LEFTY2 Monoclonal Antibody
Question
We are currently using anti-LEFTY A Monoclonal antibody M06558 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The anti-LEFTY A Monoclonal antibody (M06558) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-19
Question
I was wanting to use your anti-LEFTY A Monoclonal antibody for WB for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?
J. Johnson
Verified customer
Asked: 2019-06-05
Answer
You can see on the product datasheet, M06558 anti-LEFTY A Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-05
Question
you antibody to test anti-LEFTY A Monoclonal antibody M06558 on human uterus for research purposes, then I may be interested in using anti-LEFTY A Monoclonal antibody M06558 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
The products we sell, including anti-LEFTY A Monoclonal antibody M06558, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-30
