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Product Info Summary
| SKU: | PB9657 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LCAT Antibody Picoband®
SKU/Catalog Number
PB9657
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LCAT Antibody Picoband® catalog # PB9657. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LCAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9657)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse LCAT, different from the related human sequence by six amino acids, and from the related rat sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9657 is reactive to Lcat in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
49747 MW
Background of LCAT
LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed 'reverse cholesterol transport.' The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9657 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Testis Tissue, HEPG2 Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LCAT using anti-LCAT antibody (PB9657).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Testis Tissue Lysate at 50ug,
Lane 3: HEPG2 Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCAT antigen affinity purified polyclonal antibody (Catalog # PB9657) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCAT at approximately 50 kDa. The expected band size for LCAT is at 50 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Lcat (Source: Uniprot.org, NCBI)
Gene Name
Lcat
Full Name
Phosphatidylcholine-sterol acyltransferase
Weight
49747 MW
Superfamily
AB hydrolase superfamily
Alternative Names
Phosphatidylcholine-sterol acyltransferase;2.3.1.43 ;Lecithin-cholesterol acyltransferase;Phospholipid-cholesterol acyltransferase;Lcat; LCAT lecithin-cholesterol acyltransferase phosphatidylcholine-sterol acyltransferase|1-alkyl-2-acetylglycerophosphocholine esterase|PAF acetylhydrolase|phosphatidylcholine--sterol O-acyltransferase|phospholipid-cholesterol acyltransferase|platelet-activating factor acetylhydrolase|testicular secretory protein Li 24
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lcat, check out the Lcat Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lcat: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LCAT Antibody Picoband® (PB9657)
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Customer Q&As
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1 Customer Q&As for Anti-LCAT Antibody Picoband®
Question
We are currently using anti-LCAT antibody PB9657 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
The anti-LCAT antibody (PB9657) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-25
