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Product Info Summary
| SKU: | M01524 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody
SKU/Catalog Number
M01524
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody catalog # M01524. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01524)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IEC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LC3B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01524 is reactive to MAP1LC3B in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
18 kDa
Calculated molecular weight
14688 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01524 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human A549 whole cell, human HepG2 whole cell, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue
IHC: Human pituitary tumor tissue, Human testis cancer tissue, Human esophageal carcinoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LC3B using anti-LC3B antibody (M01524).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LC3B antigen affinity purified monoclonal antibody (Catalog # M01524) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LC3B at approximately 18 kDa. The expected band size for LC3B is at 16 kDa.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis of Hela cells treated with choroquine, using LC3B Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MAP1LC3B (Source: Uniprot.org, NCBI)
Gene Name
MAP1LC3B
Full Name
Microtubule-associated proteins 1A/1B light chain 3B
Weight
14688 MW
Superfamily
ATG8 family
Alternative Names
Microtubule-associated proteins 1A/1B light chain 3B;Autophagy-related protein LC3 B;Autophagy-related ubiquitin-like modifier LC3 B;MAP1 light chain 3-like protein 2;MAP1A/MAP1B light chain 3 B;MAP1A/MAP1B LC3 B;Microtubule-associated protein 1 light chain 3 beta;MAP1LC3B;MAP1ALC3; Map1lc3b|LC3B, Map1lc3, Mpl3, zbs559|microtubule-associated protein 1 light chain 3 beta|microtubule-associated proteins 1A/1B light chain 3B|MAP1 light chain 3-like protein 2|MAP1A/1B light chain 3 B|MAP1A/MAP1B LC3 B|MAP1A/MAP1B light chain 3 B|autophagy-related protein LC3 B|autophagy-related ubiquitin-like modifier LC3 B|microtubule-associated proteins 1A/1B light chain 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP1LC3B, check out the MAP1LC3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP1LC3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody (M01524)
Hello CJ!
M01524 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Tao,Xiaoqing Zhou,Yan Zhang,Wenfeng Pu,Yi Yang,Fuxia Wei,Qian Zhou,Lin Zhang,Zhonghan Du,Ji Wu,"Xi Lei San Attenuates Dextran Sulfate Sodium-Induced Colitis in Rats and TNF-α-Stimulated Colitis in CACO2 Cells: Involvement of the NLRP3 Inflammasome and Autophagy",Mediators of Inflammation,vol. 2021, Article ID 1610251,12 pages,2021.https://doi.org/10.1155/2021/1610251
Species: Human,Rat
M01524 usage in article: APP:WB, SAMPLE:COLON TISSUE AND CACO2 CELL, DILUTION:1:800
Xiaodong Zhou, Hongsheng Ouyang, Daxin Pang et al. Pathological Alterations in the Gastrointestinal Tract of a Porcine Model of DMD, 08 March 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-267849/v1]
Species: Piglets
M01524 usage in article: APP:WB, SAMPLE:SERUM, DILUTION:1:400
Overendocytosis of superparamagnetic iron oxide particles increases apoptosis and triggers autophagic cell death in human osteosarcoma cell under a spinning magnetic field
Dai L, Chen X, Lu X, Wang F, Zhan Y, Song G, Hu T, Xia C, Zhang B. Sci Rep. 2017 Oct 24;7(1):13912. doi: 10.1038/s41598-017-13334-y. Phosphoinositide-specific phospholipase Cγ1 inhibition induces autophagy in human colon cancer and hepatocellular ...
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