Anti-Docking protein 4 DOK4 Antibody

DOK4 antibody

Boster Bio Anti-Docking protein 4 DOK4 Antibody (Catalog # A11549). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A11549
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Docking protein 4 DOK4 Antibody

View all DOK4 Antibodies

SKU/Catalog Number

A11549

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Docking protein 4 DOK4 Antibody (Catalog # A11549). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Docking protein 4 DOK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11549)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human DOK4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11549 is reactive to DOK4 in Human, Mouse

Reconstitution

Calculated molecular weight

37028 MW

Background of DOK4

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway By similarity. Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.

Favre C., Genes Immun. 4:40-45(2003).
Cai D., J. Biol. Chem. 278:25323-25330(2003).
Loftus B.J., Genomics 60:295-308(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11549 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For DOK4 (Source: Uniprot.org, NCBI)

Gene Name

DOK4

Full Name

Docking protein 4

Weight

37028 MW

Superfamily

DOK family

Alternative Names

Docking protein 4;Downstream of tyrosine kinase 4;Insulin receptor substrate 5;IRS-5;IRS5;DOK4; DOK4 IRS-5, IRS5 docking protein 4 docking protein 4|downstream of tyrosine kinase 4|insulin receptor substrate 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DOK4, check out the DOK4 Infographic

DOK4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DOK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Docking protein 4 DOK4 Antibody

Question

I have a question about product A11549, anti-DOK4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11549 anti-DOK4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-04

Question

I am interested in to test anti-DOK4 antibody A11549 on mouse colon pancreas for research purposes, then I may be interested in using anti-DOK4 antibody A11549 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

The products we sell, including anti-DOK4 antibody A11549, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-25

Question

We are currently using anti-DOK4 antibody A11549 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

The anti-DOK4 antibody (A11549) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-15

Order DetailsPrice
A11549-100uL

100uL

$360

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A11549
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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