Anti-LATS1/2 Antibody

Product Name

Anti-LATS1/2 Antibody

View all LATS1 Antibodies

SKU/Catalog Number

A01051

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-LATS1/2 Antibody (Catalog # A01051). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LATS1/2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01051)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from Internal of human LATS1/2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01051 is reactive to LATS1 in Human

Reconstitution

Calculated molecular weight

126870 MW

Background of LATS1

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

Tao W., Nat. Genet. 21:177-181(1999).
Nishiyama Y., FEBS Lett. 459:159-165(1999).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01051 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For LATS1 (Source: Uniprot.org, NCBI)

Alternative Names

Serine/threonine-protein kinase LATS1;2.7.11.1;Large tumor suppressor homolog 1;WARTS protein kinase;h-warts;LATS1 ;WARTS ; LATS1 WARTS, wts large tumor suppressor kinase 1 serine/threonine-protein kinase LATS1|LATS (large tumor suppressor, Drosophila) homolog 1|LATS, large tumor suppressor, homolog 1|WARTS protein kinase|h-warts|large tumor suppressor homolog 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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