Anti-LAMP2 Monoclonal Antibody

Product Name

Anti-LAMP2 Monoclonal Antibody

View all LAMP-2/CD107b Antibodies

SKU/Catalog Number

M01573-1

Size

100ug

Form

liquid

Description

Boster Bio Anti-LAMP2 Monoclonal Antibody catalog # M01573-1. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LAMP2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01573-1)

Host

Rat

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

GL2A7

Isotype

IgG1

Immunogen

Purified preparation of mouse liver lysosomal membranes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~100-110kDa.

Reactive Species

M01573-1 is reactive to LAMP2 in Human, Mouse, Rabbit

Reconstitution

Calculated molecular weight

44.961kDa

Background of LAMP-2/CD107b

Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01573-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For LAMP2 (Source: Uniprot.org, NCBI)

Alternative Names

CD107b, Igp110, Igp2, Lamp2C, LampB, MAC3, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, Lysosomal membrane glycoprotein type B, LGP-B LAMP2 CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110 lysosomal associated membrane protein 2 lysosome-associated membrane glycoprotein 2|CD107 -like family member B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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