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Product Info Summary
| SKU: | PB9142 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, IHC-F, WB |
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Product info
Product Name
Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®
View all Laminin gamma 1 Antibodies
SKU/Catalog Number
PB9142
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® catalog # PB9142. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9142)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Peptide mixture of laminin gamma1,2,3 (DLNKLNEIEGTLNKAKDEMK; MSELEERARQQRGHLHLLET; LQRKLSLLEQESQQQELQIQ). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9142 is reactive to Lamc1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
177 kDa
Calculated molecular weight
177298 MW
Background of Laminin gamma 1
Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an α-chain, a β-chain, and a γ-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9142 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Positive Control
WB: human A431 whole cell, human A549 whole cell, human PC-3 whole cell, human Caco-2 whole cellrat NRK whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell, mouse Hepa1-6 whole cell
IHC: human lung cancer tissue
IHC-F: human placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Laminin using anti-Laminin antibody (PB9142).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
Lane 5: rat NRK whole cell lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody (Catalog # PB9142) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin at approximately 220-250 kDa. The expected band size for Laminin is at 177 kDa.
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Figure 2. IHC analysis of Laminin using anti-Laminin antibody (PB9142).
Laminin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Laminin Antibody (PB9142) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Laminin using anti-Laminin antibody (PB9142).
Laminin was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Laminin Antibody (PB9142) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Lamc1 (Source: Uniprot.org, NCBI)
Gene Name
Lamc1
Full Name
Laminin subunit gamma-1
Weight
177298 MW
Alternative Names
Laminin subunit gamma-1;Laminin B2 chain;Laminin-1 subunit gamma;Laminin-10 subunit gamma;Laminin-11 subunit gamma;Laminin-2 subunit gamma;Laminin-3 subunit gamma;Laminin-4 subunit gamma;Laminin-6 subunit gamma;Laminin-7 subunit gamma;Laminin-8 subunit gamma;Laminin-9 subunit gamma;S-laminin subunit gamma;S-LAM gamma;Lamc1;Lamb-2, Lamc-1; LAMC1 LAMB2 laminin subunit gamma 1 laminin subunit gamma-1|S-LAM gamma|S-laminin subunit gamma|laminin B2 chain|laminin, gamma 1 (formerly LAMB2)|laminin-10 subunit gamma|laminin-11 subunit gamma|laminin-2 subunit gamma|laminin-3 subunit gamma|laminin-4 subunit gamma|laminin-6 subunit gamma|laminin-7 subunit gamma|laminin-8 subunit gamma|laminin-9 subunit gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lamc1, check out the Lamc1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lamc1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® (PB9142)
Hello CJ!
PB9142 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Biocompatibility of pure titanium modified by human endothelial cell-derived extracellular matrix
Remarkable preservation of Ca(2+) homeostasis and inhibition of apoptosis contribute to anti-muscle atrophy effect in hibernating Daurian ground squirrels
Guo C, Xu L, He Q, Liang T, Duan X, Li R. Food Chem Toxicol. 2013 Jun;56:436-42. Doi: 10.1016/J.Fct.2013.02.051. Epub 2013 Mar 14. Anti-Fibrotic Effects Of Puerarin On Ccl4-Induced Hepatic Fibrosis In Rats Possibly Through The Regulation Of Ppar-?...
Liang S, Li Hc, Wang Yx, Wu Ss, Cai Yj, Cui Hl, Yang Yp, Ya J. Dev Growth Differ. 2014 May;56(4):276-92. Doi: 10.1111/Dgd.12129. Epub 2014 Apr 3. Pulmonary Endoderm, Second Heart Field And The Morphogenesis Of Distal Outflow Tract In Mouse Embryon...
Gao S, Zheng Y, Cai Q, Yao W, Wang J, Zhang P, Wang X. J Mater Sci Mater Med. 2014 May;25(5):1283-91. Doi: 10.1007/S10856-014-5150-3. Epub 2014 Jan 23. Comparison Of Morphology And Biocompatibility Of Acellular Nerve Scaffolds Processed By Differe...
Li T, Yang Z, Cai Hj, Song Lw, Lu Ky, Zhou Z, Wu Zd. World J Gastroenterol. 2010 Feb 14;16(6):749-54. Effects Of In Vitro Cultivated Calculus Bovis Compound On Pulmonary Lesions In Rabbits With Schistosomiasis.
Zhu B, Xu T, Yuan J, Guo X, Liu D. Plos One. 2013 Sep 19;8(9):E76282. Doi: 10.1371/Journal.Pone.0076282. Ecollection 2013. Transcriptome Sequencing Reveals Differences Between Primary And Secondary Hair Follicle-Derived Dermal Papilla Cells Of The...
Chen W, Mo W, Sun K, Huang X, Zhang Yl, Song Hy. Curr Eye Res. 2009 Dec;34(12):1057-64. Doi: 10.3109/02713680903308487. Microplasmin Degrades Fibronectin And Laminin At Vitreoretinal Interface And Outer Retina During Enzymatic Vitrectomy.
Chen Tl, Wang Hl, Liu Yh, Fang Y, Tan Rz, Zhou Ph, Zhou Q, Lv Xy. Scientificworldjournal. 2014;2014:682189. Doi: 10.1155/2014/682189. Epub 2014 Jul 14. Highly Effective Ex Vivo Gene Manipulation To Study Kidney Development Using Self-Complementary...
Cheng Y, Wang Y, Kang Yz, Hu Py, Gao Y, Pan Mx. Digestion. 2013;87(3):189-95. Doi: 10.1159/000349949. Epub 2013 May 1. In Vitro Culture Of Tumour-Derived Hepatocytes In Decellularised Whole-Liver Biological Scaffolds.
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Customer Q&As
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1 Customer Q&As for Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®
Question
I was wanting to use using your anti-Laminin/Lamc1/Lamc2/Lamc3 antibody for hemidesmosome assembly studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you for your inquiry. This PB9142 anti-Laminin/Lamc1/Lamc2/Lamc3 antibody is tested on hela whole cell lysate, hepg2 whole cell lysate, lung cancer tissue. It is guaranteed to work for IHC-P, IHC-F, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-04
