Product Info Summary
SKU: | M00438-5 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Lamin A/C Rabbit Monoclonal Antibody
SKU/Catalog Number
M00438-5
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Lamin A/C Rabbit Monoclonal Antibody catalog # M00438-5. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lamin A/C Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00438-5)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22L53
Isotype
IgG
Immunogen
A synthesized peptide derived from human Lamin A/C
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00438-5 is reactive to LMNA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70-74 kDa
Calculated molecular weight
74139 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00438-5 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LaminA/C using anti-LaminA/C antibody (M00438-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LaminA/C antigen affinity purified monoclonal antibody (Catalog # M00438-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LaminA/C at approximately 70-74 kDa. The expected band size for LaminA/C is at 74 kDa.
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For LMNA (Source: Uniprot.org, NCBI)
Gene Name
LMNA
Full Name
Prelamin-A/C
Weight
74139 MW
Superfamily
intermediate filament family
Alternative Names
Prelamin-A/C;Lamin-A/C;70 kDa lamin;Renal carcinoma antigen NY-REN-32;LMNA;LMN1; LMNA CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1 lamin A/C lamin|70 kDa lamin|epididymis secretory sperm binding protein|lamin A/C-like 1|mandibuloacral dysplasia type A|prelamin-A/C|renal carcinoma NY-REN-32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMNA, check out the LMNA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lamin A/C Rabbit Monoclonal Antibody (M00438-5)
Hello CJ!
M00438-5 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Medical findings of nasopharyngeal carcinoma patients and anti-tumor benefits of formononetin
NBPF is a potential DNA-binding transcription factor that is directly regulated by NF-κB
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