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Product Info Summary
| SKU: | M00438-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | IF, WB |
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Product info
Product Name
Anti-Lamin A/C LMNA Antibody
SKU/Catalog Number
M00438-2
Size
100ul
Description
Boster Bio Anti-Lamin A/C LMNA Antibody catalog # M00438-2. Tested in IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Stable at 4℃ for one year.
Cite This Product
Anti-Lamin A/C LMNA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00438-2)
Host
Chicken
Contents
Supplied as an aliquot of concentrated IgY preparation plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Full length recombinant human lamin A protein expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00438-2 is reactive to LMNA in Human, Mouse, Rat
Applications
M00438-2 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
74139 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2,000. IF/ICC 1:1,000
Validation Images & Assay Conditions
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Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken pAb to lamin A/C, M00438-2, dilution 1:1,000 in green: [1] protein standard (red), [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 74 and 65kDa correspond to lamin A and lamin C proteins respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse mAb to GAPDH, in red. The single band at 37kDa represents GAPDH protein which is expressed predominantly in the cytosolic fractions.
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Immunofluorescent analysis of HeLa cells stained with chicken pAb to lamin A/C, M00438-2, dilution 1:2,000 in red, and costained with mouse mAb to actin, dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. The M00438-2 antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.
Protein Target Info & Infographic
Gene/Protein Information For LMNA (Source: Uniprot.org, NCBI)
Gene Name
LMNA
Full Name
Prelamin-A/C
Weight
74139 MW
Superfamily
intermediate filament family
Alternative Names
CDCD1; CDDC; CMD1A; CMT2B1; dilated 1A (autosomal dominant); EMD2; FPLD; HGPSFPL; lamin A/C; lamin A/C-like 1; lamin-A/C; LDP1; LFP; LGMD1B; limb girdle muscular dystrophy 1B (autosomal dominant); LMN1IDC; LMNC; LMNL1; prelamin-A/C; PRO1,70 kDa lamin; progeria 1 (Hutchinson-Gilford type); renal carcinoma antigen NY-REN-32 LMNA CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1 lamin A/C lamin|70 kDa lamin|epididymis secretory sperm binding protein|lamin A/C-like 1|mandibuloacral dysplasia type A|prelamin-A/C|renal carcinoma antigen NY-REN-32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMNA, check out the LMNA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lamin A/C LMNA Antibody (M00438-2)
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No publications found for M00438-2
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Lamin A/C LMNA Antibody
Question
Please see the WB image, lot number and protocol we used for lung skin using anti-Lamin A/C antibody M00438-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-07
Question
I am interested in to test anti-Lamin A/C antibody M00438-2 on mouse lung skin for research purposes, then I may be interested in using anti-Lamin A/C antibody M00438-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The products we sell, including anti-Lamin A/C antibody M00438-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-27
Question
I was wanting to use your anti-Lamin A/C antibody for IF for mouse lung skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung skin identification?
E. Moore
Verified customer
Asked: 2020-02-17
Answer
As indicated on the product datasheet, M00438-2 anti-Lamin A/C antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-17
Question
I see that the anti-Lamin A/C antibody M00438-2 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-06
Question
We were happy with the WB result of your anti-Lamin A/C antibody. However we have been able to see positive staining in liver nucleus. using this antibody. Is that expected? Could you tell me where is LMNA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Based on literature, liver does express LMNA. Generally LMNA expresses in nucleus., isoform c: nucleus speckle. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485
Boster Scientific Support
Answered: 2019-11-18
Question
Would anti-Lamin A/C antibody M00438-2 work for IF with lung skin?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to the expression profile of lung skin, LMNA is highly expressed in lung skin. So, it is likely that anti-Lamin A/C antibody M00438-2 will work for IF with lung skin.
Boster Scientific Support
Answered: 2019-08-30
Question
We purchased anti-Lamin A/C antibody for WB on renal cell carcinoma a few months ago. I am using rat, and We intend to use the antibody for IF next. We need examining renal cell carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
I viewed the website and datasheets of our anti-Lamin A/C antibody and it seems that M00438-2 has been tested on rat in both WB and IF. Thus M00438-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-05
Question
Will M00438-2 anti-Lamin A/C antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Jha
Verified customer
Asked: 2019-04-03
Answer
It shows on the product datasheet, M00438-2 anti-Lamin A/C antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-03
Question
I would like using your anti-Lamin A/C antibody for negative regulation of cell population proliferation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
N. Collins
Verified customer
Asked: 2018-12-21
Answer
Thank you for your inquiry. This M00438-2 anti-Lamin A/C antibody is tested on hela cells. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung skin using anti-Lamin A/C antibody M00438-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-13
Question
Is there a BSA free version of anti-Lamin A/C antibody M00438-2 available?
P. Gonzalez
Verified customer
Asked: 2018-05-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Lamin A/C antibody M00438-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-04
Question
We have been able to see staining in mouse corpus callosum. Are there any suggestions? Is anti-Lamin A/C antibody supposed to stain corpus callosum positively?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
From literature corpus callosum does express LMNA. From Uniprot.org, LMNA is expressed in mucosa of stomach, corpus callosum, kidney, lung skin, ovarian carcinoma, colon, renal cell carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485
Boster Scientific Support
Answered: 2017-08-30
Question
Is this M00438-2 anti-Lamin A/C antibody reactive to the isotypes of LMNA?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
The immunogen of M00438-2 anti-Lamin A/C antibody is Full length recombinant human lamin A protein expressed in and purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-07
Question
My question regarding product M00438-2, anti-Lamin A/C antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Jackson
Verified customer
Asked: 2016-01-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00438-2 anti-Lamin A/C antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-01-08
Question
Is a blocking peptide available for product anti-Lamin A/C antibody (M00438-2)?
E. Gonzalez
Verified customer
Asked: 2013-12-10
Answer
We do provide the blocking peptide for product anti-Lamin A/C antibody (M00438-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-12-10
Question
We are currently using anti-Lamin A/C antibody M00438-2 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
S. Li
Verified customer
Asked: 2013-06-19
Answer
The anti-Lamin A/C antibody (M00438-2) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-19
