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Product Info Summary
| SKU: | M00438 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Lamin A/C LMNA Rabbit Monoclonal Antibody
SKU/Catalog Number
M00438
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Lamin A/C LMNA Rabbit Monoclonal Antibody catalog # M00438. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lamin A/C LMNA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00438)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BEO-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Lamin A/C
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00438 is reactive to LMNA in Human
Applications
M00438 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
160-180 kDa
Calculated molecular weight
74139 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:3000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Lamin A/C Antibody.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Western blot analysis of Lamin A/C expression in HeLa whole cell lysates.
Protein Target Info & Infographic
Gene/Protein Information For LMNA (Source: Uniprot.org, NCBI)
Gene Name
LMNA
Full Name
Prelamin-A/C
Weight
74139 MW
Superfamily
intermediate filament family
Alternative Names
CDCD1; CDDC; CMD1A; CMT2B1; dilated 1A (autosomal dominant); EMD2; FPLD; HGPSFPL; lamin A/C; lamin A/C-like 1; lamin-A/C; LDP1; LFP; LGMD1B; limb girdle muscular dystrophy 1B (autosomal dominant); LMN1IDC; LMNC; LMNL1; prelamin-A/C; PRO1,70 kDa lamin; progeria 1 (Hutchinson-Gilford type); renal carcinoma antigen NY-REN-32 LMNA CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1 lamin A/C lamin|70 kDa lamin|epididymis secretory sperm binding protein|lamin A/C-like 1|mandibuloacral dysplasia type A|prelamin-A/C|renal carcinoma antigen NY-REN-32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMNA, check out the LMNA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lamin A/C LMNA Rabbit Monoclonal Antibody (M00438)
Hello CJ!
M00438 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
714: Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Lamin A/C LMNA Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Lamin A/C Rabbit Monoclonal antibody for IP for human ovarian carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovarian carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
You can see on the product datasheet, M00438 anti-Lamin A/C Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ovarian carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-22
Question
Is this M00438 anti-Lamin A/C Rabbit Monoclonal antibody reactive to the isotypes of LMNA?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The immunogen of M00438 anti-Lamin A/C Rabbit Monoclonal antibody is A synthesized peptide derived from human Lamin A/C. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-16
Question
Will anti-Lamin A/C Rabbit Monoclonal antibody M00438 work for IP with ovarian carcinoma?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
According to the expression profile of ovarian carcinoma, LMNA is highly expressed in ovarian carcinoma. So, it is likely that anti-Lamin A/C Rabbit Monoclonal antibody M00438 will work for IP with ovarian carcinoma.
Boster Scientific Support
Answered: 2020-03-23
Question
I see that the anti-Lamin A/C Rabbit Monoclonal antibody M00438 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-02
Question
Will M00438 anti-Lamin A/C Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
As indicated on the product datasheet, M00438 anti-Lamin A/C Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-20
Question
I have a question about product M00438, anti-Lamin A/C Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00438 anti-Lamin A/C Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-31
Question
We ordered your anti-Lamin A/C Rabbit Monoclonal antibody for IF on ovarian carcinoma in a previous experiment. I am using human, and I plan to use the antibody for WB next. We are interested in examining ovarian carcinoma as well as renal cell carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
I have checked the website and datasheets of our anti-Lamin A/C Rabbit Monoclonal antibody and it seems that M00438 has been tested on human in both IF and WB. Thus M00438 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-Lamin A/C Rabbit Monoclonal antibody M00438 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The anti-Lamin A/C Rabbit Monoclonal antibody (M00438) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovarian carcinoma using anti-Lamin A/C Rabbit Monoclonal antibody M00438. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-05
Question
Is a blocking peptide available for product anti-Lamin A/C Rabbit Monoclonal antibody (M00438)?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
We do provide the blocking peptide for product anti-Lamin A/C Rabbit Monoclonal antibody (M00438). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-15
Question
I have attached the WB image, lot number and protocol we used for ovarian carcinoma using anti-Lamin A/C Rabbit Monoclonal antibody M00438. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
We have been able to see staining in human corpus callosum. Are there any suggestions? Is anti-Lamin A/C Rabbit Monoclonal antibody supposed to stain corpus callosum positively?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
According to literature corpus callosum does express LMNA. According to Uniprot.org, LMNA is expressed in mucosa of stomach, corpus callosum, kidney, lung skin, ovarian carcinoma, colon, renal cell carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485
Boster Scientific Support
Answered: 2019-06-07
Question
We were content with the WB result of your anti-Lamin A/C Rabbit Monoclonal antibody. However we have been able to see positive staining in mucosa of stomach isoform c: nucleus speckle using this antibody. Is that expected? Could you tell me where is LMNA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
According to literature, mucosa of stomach does express LMNA. Generally LMNA expresses in nucleus., isoform c: nucleus speckle. Regarding which tissues have LMNA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Pubmed ID: 8621584
Corpus callosum, Pubmed ID: 14702039
Kidney, Lung, and Skin, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Renal cell carcinoma, Pubmed ID: 10508479, 10514485
Boster Scientific Support
Answered: 2018-12-13
Question
My question regards using your anti-Lamin A/C Rabbit Monoclonal antibody for negative regulation of extrinsic apoptotic signaling pathway studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
P. Johnson
Verified customer
Asked: 2018-08-27
Answer
Thanks for your inquiry. This M00438 anti-Lamin A/C Rabbit Monoclonal antibody is tested on hela whole cell lysates. It is guaranteed to work for IP, IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-27
Question
My lab would like to test anti-Lamin A/C Rabbit Monoclonal antibody M00438 on human ovarian carcinoma for research purposes, then I may be interested in using anti-Lamin A/C Rabbit Monoclonal antibody M00438 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Li
Verified customer
Asked: 2015-12-09
Answer
The products we sell, including anti-Lamin A/C Rabbit Monoclonal antibody M00438, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-12-09
Question
Is there a BSA free version of anti-Lamin A/C Rabbit Monoclonal antibody M00438 available?
A. Wu
Verified customer
Asked: 2014-11-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Lamin A/C Rabbit Monoclonal antibody M00438 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-11-28
