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Product Info Summary
| SKU: | A10229 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-LAMC3/Laminin Gamma 3 Antibody
SKU/Catalog Number
A10229
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-LAMC3/Laminin Gamma 3 Antibody (Catalog # A10229). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LAMC3/Laminin Gamma 3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10229)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human LAMC3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10229 is reactive to LAMC3 in Human, Mouse
Reconstitution
Calculated molecular weight
171227 MW
Background of LAMC3
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Koch M., J. Cell Biol. 145:605-618(1999).
Humphray S.J., Nature 429:369-374(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10229 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using LAMC3 antibody A10229.
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Western blot analysis of extracts from K562 cells and 3T3 cells, using LAMC3 antiobdy A10229.
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Immunofluorescence analysis of LOVO cells, using LAMC3 antibody A10229.
Protein Target Info & Infographic
Gene/Protein Information For LAMC3 (Source: Uniprot.org, NCBI)
Gene Name
LAMC3
Full Name
Laminin subunit gamma-3
Weight
171227 MW
Alternative Names
Laminin subunit gamma-3;Laminin-12 subunit gamma;Laminin-14 subunit gamma;Laminin-15 subunit gamma;LAMC3; LAMC3 OCCM laminin subunit gamma 3 laminin subunit gamma-3|laminin, gamma 3|laminin-12 subunit gamma|laminin-14 subunit gamma|laminin-15 subunit gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMC3, check out the LAMC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LAMC3/Laminin Gamma 3 Antibody (A10229)
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No publications found for A10229
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Customer Q&As
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6 Customer Q&As for Anti-LAMC3/Laminin Gamma 3 Antibody
Question
Is a blocking peptide available for product anti-LAMC3/Laminin Gamma 3 antibody (A10229)?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do provide the blocking peptide for product anti-LAMC3/Laminin Gamma 3 antibody (A10229). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-17
Question
We are currently using anti-LAMC3/Laminin Gamma 3 antibody A10229 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
The anti-LAMC3/Laminin Gamma 3 antibody (A10229) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-08
Question
I would like to test anti-LAMC3/Laminin Gamma 3 antibody A10229 on mouse placenta for research purposes, then I may be interested in using anti-LAMC3/Laminin Gamma 3 antibody A10229 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Dhar
Verified customer
Asked: 2018-07-05
Answer
The products we sell, including anti-LAMC3/Laminin Gamma 3 antibody A10229, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-LAMC3/Laminin Gamma 3 antibody A10229. Let me know if you need anything else.
N. Wu
Verified customer
Asked: 2018-05-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-28
Question
See below the WB image, lot number and protocol we used for placenta using anti-LAMC3/Laminin Gamma 3 antibody A10229. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-13
Question
Is there a BSA free version of anti-LAMC3/Laminin Gamma 3 antibody A10229 available?
A. Brown
Verified customer
Asked: 2014-08-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LAMC3/Laminin Gamma 3 antibody A10229 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-08-18
