Product Info Summary
SKU: | M01776 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-KPNA2 Monoclonal Antibody
View all Importin alpha 2/KPNA2 Antibodies
SKU/Catalog Number
M01776
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KPNA2 Monoclonal Antibody catalog # M01776. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KPNA2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01776)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEAF-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KPNA2 Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01776 is reactive to KPNA2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
93558 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01776 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
FC 1:50
Positive Control
WB: HeLa cell, C6 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of KPNA2 expression in (1) HeLa cell lysate; (2) C6 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For KPNA2 (Source: Uniprot.org, NCBI)
Gene Name
KPNA2
Full Name
Importin subunit alpha-1
Weight
93558 MW
Superfamily
importin alpha family
Alternative Names
Axin-2;Axin-like protein;Axil;Axis inhibition protein 2;Conductin;AXIN2; KPNA2 IPOA1, QIP2, RCH1, SRP1-alpha, SRP1alpha karyopherin subunit alpha 2 importin subunit alpha-1|RAG cohort protein 1|importin subunit alpha-2|importin-alpha-P1|karyopherin alpha 2 (RAG cohort 1, importin alpha 1)|pendulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KPNA2, check out the KPNA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KPNA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KPNA2 Monoclonal Antibody (M01776)
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No publications found for M01776
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Customer Q&As
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8 Customer Q&As for Anti-KPNA2 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01776?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of KPNA2 expression in (1) HeLa cell lysate; (2) C6 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow testis using anti-KPNA2 Monoclonal antibody M01776. Let me know if you need anything else.
T. Brown
Verified customer
Asked: 2019-11-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
See attached the WB image, lot number and protocol we used for bone marrow testis using anti-KPNA2 Monoclonal antibody M01776. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-04
Question
I see that the anti-KPNA2 Monoclonal antibody M01776 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-20
Question
My lab would like to test anti-KPNA2 Monoclonal antibody M01776 on human bone marrow testis for research purposes, then I may be interested in using anti-KPNA2 Monoclonal antibody M01776 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Miller
Verified customer
Asked: 2016-08-03
Answer
The products we sell, including anti-KPNA2 Monoclonal antibody M01776, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-08-03
Question
Is there a BSA free version of anti-KPNA2 Monoclonal antibody M01776 available?
B. Miller
Verified customer
Asked: 2016-05-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-KPNA2 Monoclonal antibody M01776 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-03
Question
Is a blocking peptide available for product anti-KPNA2 Monoclonal antibody (M01776)?
G. Parker
Verified customer
Asked: 2015-04-28
Answer
We do provide the blocking peptide for product anti-KPNA2 Monoclonal antibody (M01776). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-04-28
Question
Does anti-KPNA2 Monoclonal antibody M01776 work for WB with bone marrow testis?
N. Carter
Verified customer
Asked: 2014-03-04
Answer
According to the expression profile of bone marrow testis, KPNA2 is highly expressed in bone marrow testis. So, it is likely that anti-KPNA2 Monoclonal antibody M01776 will work for WB with bone marrow testis.
Boster Scientific Support
Answered: 2014-03-04