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Product Info Summary
| SKU: | M00050 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00050
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody catalog # M00050. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00050)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOOO-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KMT6 / EZH2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00050 is reactive to EZH2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
85363 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00050 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of KMT6 / EZH2 expression in Hela cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For EZH2 (Source: Uniprot.org, NCBI)
Gene Name
EZH2
Full Name
Histone-lysine N-methyltransferase EZH2
Weight
85363 MW
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
Histone-lysine N-methyltransferase EZH2;2.1.1.43;ENX-1;Enhancer of zeste homolog 2;Lysine N-methyltransferase 6;EZH2;KMT6; EZH2 ENX-1, ENX1b, KMT6, KMT6A, WVS, WVS2, EZH2 enhancer of zeste 2 polycomb repressive complex 2 subunit histone-lysine N-methyltransferase EZH2|enhancer of zeste homolog 2|lysine N-methyltransferase 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EZH2, check out the EZH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EZH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody (M00050)
Hello CJ!
M00050 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Enhancer of zeste homolog 2 silencing inhibits tumor growth and lung metastasis in osteosarcoma
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody
Question
I am looking for using your anti-KMT6 / EZH2 Rabbit Monoclonal antibody for rhythmic process studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
We appreciate your inquiry. This M00050 anti-KMT6 / EZH2 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-05
Question
Our team were content with the WB result of your anti-KMT6 / EZH2 Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleus using this antibody. Is that expected? Could you tell me where is EZH2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
According to literature, cervix carcinoma does express EZH2. Generally EZH2 expresses in nucleus. Regarding which tissues have EZH2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8954776
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-10
Question
We have observed staining in human cervix carcinoma erythroleukemia. Do you have any suggestions? Is anti-KMT6 / EZH2 Rabbit Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
According to literature cervix carcinoma erythroleukemia does express EZH2. According to Uniprot.org, EZH2 is expressed in lung, brain, brain testis, pancreas, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have EZH2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8954776
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-12
Question
We are currently using anti-KMT6 / EZH2 Rabbit Monoclonal antibody M00050 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
P. Rodriguez
Verified customer
Asked: 2013-05-10
Answer
The anti-KMT6 / EZH2 Rabbit Monoclonal antibody (M00050) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-10
