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Product Info Summary
| SKU: | M00840 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KMT4 / Dot1L Rabbit Monoclonal Antibody
SKU/Catalog Number
M00840
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KMT4 / Dot1L Rabbit Monoclonal Antibody catalog # M00840. Tested in WB application. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KMT4 / Dot1L Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00840)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CBF-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KMT4 / Dot1L
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00840 is reactive to DOT1L in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
184853 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00840 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:3000
Positive Control
WB: Raw2647 cell
Validation Images & Assay Conditions
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Western blot analysis of KMT4/Dot1L expression in RAW264.7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DOT1L (Source: Uniprot.org, NCBI)
Gene Name
DOT1L
Full Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Weight
184853 MW
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
Histone-lysine N-methyltransferase, H3 lysine-79 specific;2.1.1.43;DOT1-like protein;Histone H3-K79 methyltransferase;H3-K79-HMTase;Lysine N-methyltransferase 4;DOT1L;KIAA1814, KMT4; DOT1L DOT1, KMT4 DOT1 like histone lysine methyltransferase histone-lysine N-methyltransferase, H3 lysine-79 specific|DOT1 like histone H3K79 methyltransferase|DOT1-like histone methyltransferase|DOT1-like protein|DOT1-like, histone H3 methyltransferase|H3-K79-HMTase|histone H3-K79 methyltransferase|histone methyltransferase DOT1L|lysine N-methyltransferase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DOT1L, check out the DOT1L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DOT1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KMT4 / Dot1L Rabbit Monoclonal Antibody (M00840)
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Customer Q&As
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1 Customer Q&As for Anti-KMT4 / Dot1L Rabbit Monoclonal Antibody
Question
We are currently using anti-KMT4 / Dot1L Rabbit Monoclonal antibody M00840 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
J. Rodriguez
Verified customer
Asked: 2019-10-03
Answer
The anti-KMT4 / Dot1L Rabbit Monoclonal antibody (M00840) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
