Anti-KLF10 Antibody Picoband™

Product Name

Anti-KLF10 Antibody Picoband™

View all KLF10 Antibodies

SKU/Catalog Number

A03419-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KLF10 Antibody Picoband™ catalog # A03419-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-KLF10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03419-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human KLF10 recombinant protein (Position: K36-A471).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03419-2 is reactive to KLF10 in Human

Applications

A03419-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

73109 MW

Background of KLF10

Krueppel-like factor 10 is a protein that in humans is encoded by the KLF10 gene. This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of KLF10 using anti-KLF10 antibody (A03419-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF10 antigen affinity purified polyclonal antibody (Catalog # A03419-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF10 at approximately 55 kDa. The expected band size for KLF10 is at 53 kDa.

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Figure 2. IF analysis of KLF10 using anti-KLF10 antibody (A03419-2).
KLF10 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-KLF10 Antibody (A03419-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For KLF10 (Source: Uniprot.org, NCBI)

Alternative Names

EGRA; EGR-alpha; KLF10; Krueppel-like factor 10; Kruppel-like factor 10; TGFB inducible early growth response; TGFB-inducible early growth response protein 1; TIEG1; TIEG-1; TIEG1TIEG; Transforming growth factor-beta-inducible early growth response protein 1 KLF10 EGR-alpha, EGRA, TIEG, TIEG1 Kruppel like factor 10 Krueppel-like factor 10|TGFB-inducible early growth response protein 1|early growth response-alpha|transforming growth factor-beta-inducible early growth response protein 1|zinc finger transcription factor TIEG

For more info on KLF10, check out the KLF10 Infographic

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In this infographic, you will see the following information for KLF10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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