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Product Info Summary
| SKU: | M00689-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-KISS1 Antibody (C-Term)
SKU/Catalog Number
M00689-1
Size
80 µl
Description
Boster Bio Anti-KISS1 Antibody (C-Term) (Catalog # M00689-1). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KISS1 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00689-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This KISS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the human region of human KISS1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00689-1 is reactive to KISS1 in Human
Reconstitution
Calculated molecular weight
14705 Da
Background of KISS1
Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell- matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine- tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00689-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling KISS1 with M00689-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-KISS1 Antibody (C-Term) at 1:2000 dilution
Lane 1: HL-60 whole cell lysates
Lane 2: SW480 whole cell lysates
Lane 3: Jurkat whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-KISS1 Antibody (C-Term)at 1:2000 dilution + HL-60 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For KISS1 (Source: Uniprot.org, NCBI)
Gene Name
KISS1
Full Name
Metastasis-suppressor KiSS-1
Weight
14705 Da
Superfamily
KISS1 family
Alternative Names
Metastasis-suppressor KiSS-1, Kisspeptin-1, Metastin, Kisspeptin-54, Kisspeptin-14, Kisspeptin-13, Kisspeptin-10, KISS1 KISS1 HH13, KiSS-1 KiSS-1 metastasis suppressor metastasis-suppressor KiSS-1|kisspeptin-1|malignant melanoma metastasis-suppressor|metastin|prepro-kisspeptin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KISS1, check out the KISS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KISS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KISS1 Antibody (C-Term) (M00689-1)
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