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Product Info Summary
| SKU: | A10858-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KIR5.1 KCNJ16 Antibody
SKU/Catalog Number
A10858-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KIR5.1 KCNJ16 Antibody catalog # A10858-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KIR5.1 KCNJ16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10858-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from mouse Kir5.1. AA range:369-418
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10858-1 is reactive to KCNJ16 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
47949 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10858-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using KIR5.1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For KCNJ16 (Source: Uniprot.org, NCBI)
Gene Name
KCNJ16
Full Name
Inward rectifier potassium channel 16
Weight
47949 MW
Superfamily
inward rectifier-type potassium channel (TC 1.A.2.1) family
Alternative Names
Inward rectifier potassium channel 16;Inward rectifier K (+) channel Kir5.1;Potassium channel, inwardly rectifying subfamily J member 16;KCNJ16; KCNJ16 BIR9, KIR5.1 potassium inwardly rectifying channel subfamily J member 16 inward rectifier potassium channel 16|inward rectifier K(+) channel Kir5.1|inward rectifier K+ channel KIR5.1|potassium channel, inwardly rectifying subfamily J member 16|potassium voltage-gated channel subfamily J member 16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNJ16, check out the KCNJ16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNJ16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KIR5.1 KCNJ16 Antibody (A10858-1)
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Customer Q&As
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7 Customer Q&As for Anti-KIR5.1 KCNJ16 Antibody
Question
Is this A10858-1 anti-KIR5.1 antibody reactive to the isotypes of KCNJ16?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The immunogen of A10858-1 anti-KIR5.1 antibody is Synthesized peptide derived from mouse KIR5.1 around the non-phosphorylation site of S416. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-KIR5.1 antibody A10858-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-KIR5.1 antibody (A10858-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Would anti-KIR5.1 antibody A10858-1 work on pig WB with kidney parathyroid?
B. Moore
Verified customer
Asked: 2019-11-01
Answer
Our lab technicians have not tested anti-KIR5.1 antibody A10858-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-KIR5.1 antibody A10858-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig kidney parathyroid in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
Would anti-KIR5.1 antibody A10858-1 work for WB with adult mammalian kidney?
J. Jones
Verified customer
Asked: 2019-10-16
Answer
According to the expression profile of adult mammalian kidney, KCNJ16 is highly expressed in adult mammalian kidney. So, it is likely that anti-KIR5.1 antibody A10858-1 will work for WB with adult mammalian kidney.
Boster Scientific Support
Answered: 2019-10-16
Question
I was wanting to use your anti-KIR5.1 antibody for WB for rat adult mammalian kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat adult mammalian kidney identification?
S. Mitchell
Verified customer
Asked: 2017-04-19
Answer
It shows on the product datasheet, A10858-1 anti-KIR5.1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat adult mammalian kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-19
Question
Does A10858-1 anti-KIR5.1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Wu
Verified customer
Asked: 2015-04-08
Answer
It shows on the product datasheet, A10858-1 anti-KIR5.1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-04-08
Question
Is a blocking peptide available for product anti-KIR5.1 antibody (A10858-1)?
A. Parker
Verified customer
Asked: 2013-02-25
Answer
We do provide the blocking peptide for product anti-KIR5.1 antibody (A10858-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-02-25
