Anti-KIF3A Antibody Picoband™

Product Name

Anti-KIF3A Antibody Picoband™

View all KIF3A Antibodies

SKU/Catalog Number

PB9654

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KIF3A Antibody Picoband™ catalog # PB9654. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KIF3A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9654)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human KIF3A recombinant protein (Position: D485-Q699). Human KIF3A shares 97.7% amino acid (aa) sequence identity with mouse KIF3A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9654 is reactive to KIF3A in Human, Mouse, Rat

Applications

PB9654 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

80041 MW

Background of KIF3A

Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene. KIF3A is one subunit of the heterotrimeric motor protein, kinesin-2, that was initially isolated from sea urchin egg/embryo cytosol using microtubule affinity purification. This motor consists of two kinesin-related subunits (called KIF3A and KIF3B or 3C in vertebrates) and an associated protein (KAP3), and it transports protein complexes, nucleic acids and organelles towards the "plus" ends of microtubule tracks within cells. Work done in a broad range of eukaryotic cells has revealed that heterotrimeric kinesin-2 is the primary motor protein driving the intra-flagellar transport of tubulins and other axonemal building blocks from the base of the ciliary/flagellar axoneme to their site of assembly at the distal tips. This process is required for cilium assembly/maintenance and cilium-based signalling which play key roles in various cell and developmental processes. For example, in vertebrate embryos, kinesin-2 function is required for cilia-dependent nodal flow and the development of left-right asymmetry.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of KIF3A using anti-KIF3A antibody (PB9654).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Rat Testis Tissue Lysate at 50ug,
Lane 3: Mouse Brain Tissue Lysate at 50ug,
Lane 4: Mouse Testis Tissue Lysate at 50ug,
Lane 5: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF3A antigen affinity purified polyclonal antibody (Catalog # PB9654) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF3A at approximately 80 kDa. The expected band size for KIF3A is at 80 kDa.

Gene/Protein Information For KIF3A (Source: Uniprot.org, NCBI)

Alternative Names

KIF3; kinesin family member 3A; kinesin family protein 3A; kinesin-like protein KIF3A; Microtubule plus end-directed kinesin motor 3A KIF3A FLA10, KLP-20 kinesin family member 3A kinesin-like protein KIF3A|kinesin family protein 3A|microtubule plus end-directed kinesin motor 3A

For more info on KIF3A, check out the KIF3A Infographic

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In this infographic, you will see the following information for KIF3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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