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Product Info Summary
| SKU: | PB9654 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KIF3A Antibody Picoband®
SKU/Catalog Number
PB9654
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KIF3A Antibody Picoband® catalog # PB9654. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KIF3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9654)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KIF3A recombinant protein (Position: D485-Q699). Human KIF3A shares 97.7% amino acid (aa) sequence identity with mouse KIF3A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9654 is reactive to KIF3A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
80041 MW
Background of KIF3A
Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene. KIF3A is one subunit of the heterotrimeric motor protein, kinesin-2, that was initially isolated from sea urchin egg/embryo cytosol using microtubule affinity purification. This motor consists of two kinesin-related subunits (called KIF3A and KIF3B or 3C in vertebrates) and an associated protein (KAP3), and it transports protein complexes, nucleic acids and organelles towards the "plus" ends of microtubule tracks within cells. Work done in a broad range of eukaryotic cells has revealed that heterotrimeric kinesin-2 is the primary motor protein driving the intra-flagellar transport of tubulins and other axonemal building blocks from the base of the ciliary/flagellar axoneme to their site of assembly at the distal tips. This process is required for cilium assembly/maintenance and cilium-based signalling which play key roles in various cell and developmental processes. For example, in vertebrate embryos, kinesin-2 function is required for cilia-dependent nodal flow and the development of left-right asymmetry.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9654 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Rat Testis Tissue, Mouse Brain Tissue, Mouse Testis Tissue, MCF-7 Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KIF3A using anti-KIF3A antibody (PB9654).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Rat Testis Tissue Lysate at 50ug,
Lane 3: Mouse Brain Tissue Lysate at 50ug,
Lane 4: Mouse Testis Tissue Lysate at 50ug,
Lane 5: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF3A antigen affinity purified polyclonal antibody (Catalog # PB9654) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF3A at approximately 80 kDa. The expected band size for KIF3A is at 80 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KIF3A (Source: Uniprot.org, NCBI)
Gene Name
KIF3A
Full Name
Kinesin-like protein KIF3A
Weight
80041 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Kinesin-like protein KIF3A;Microtubule plus end-directed kinesin motor 3A;KIF3A;KIF3; KIF3A FLA10, KLP-20 kinesin family member 3A kinesin-like protein KIF3A|kinesin family protein 3A|microtubule plus end-directed kinesin motor 3A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIF3A, check out the KIF3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIF3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KIF3A Antibody Picoband® (PB9654)
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Customer Q&As
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1 Customer Q&As for Anti-KIF3A Antibody Picoband®
Question
We are currently using anti-KIF3A antibody PB9654 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
The anti-KIF3A antibody (PB9654) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-03
