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Product Info Summary
| SKU: | A10722 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-B9 domain-containing protein 1 B9D1 Antibody
SKU/Catalog Number
A10722
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-B9 domain-containing protein 1 B9D1 Antibody (Catalog # A10722). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
B9D1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-B9 domain-containing protein 1 B9D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10722)
Host
Rabbit
Contents
B9D1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
B9D1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human B9D1. The immunogen is located within amino acids 130 - 180 of B9D1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of B9D1 are known to exist; this antibody will only recognize the longest isoform. B9D1 antibody is predicted to not cross-react with other DNAJC family members.
Reactive Species
A10722 is reactive to B9D1 in Human
Applications
A10722 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
22775 MW
Background of B9D1
Meckel syndrome (MKS) is an embryonic lethal, autosomal recessive disorder characterized by polycystic kidney disease, central nervous system defects, polydactyly and liver fibrosis. B9D1 is a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. B9D1, and its related protein B9D2, form a complex with MKS1, disruption of which causes MKS. B9D1 is thought to be required for normal hedgehog signaling, ciliogenesis, and ciliary protein localization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
B9D1 antibody can be used for detection of B9D1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of B9D1 in 293 cell lysate with B9D1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of B9D1 in 293 cells with B9D1 antibody at 5 μg/mL.
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Immunofluorescence of B9D1 in 293 cells with B9D1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For B9D1 (Source: Uniprot.org, NCBI)
Gene Name
B9D1
Full Name
B9 domain-containing protein 1
Weight
22775 MW
Superfamily
B9D family
Alternative Names
B9 domain-containing protein 1; B9 protein domain 1; B9; endothelial precursor protein B9; EPPB9; MKS1-related protein 1; MKSR1 B9D1 B9, EPPB9, JBTS27, MKS9, MKSR-1, MKSR1 B9 domain containing 1 B9 domain-containing protein 1|B9 protein domain 1|MKS1-related protein 1|endothelial precursor protein B9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on B9D1, check out the B9D1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for B9D1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B9 domain-containing protein 1 B9D1 Antibody (A10722)
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