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Product Info Summary
| SKU: | A05570 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-KID KIF22 Monoclonal Antibody
SKU/Catalog Number
A05570
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KID KIF22 Monoclonal Antibody catalog # A05570. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KID KIF22 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05570)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1E3
Isotype
IgG
Immunogen
Purified recombinant fragment of human KID expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05570 is reactive to KIF22 in Human
Applications
A05570 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
73262 MW
Background of KIF22
DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
FC, 1:200-1:400
Validation Images & Assay Conditions
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Western Blot (WB) analysis using KID Monoclonal antibody against HEK293 (1) and KID-hIgGFc transfected HEK293 (2) cell lysate.
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Flow cytometric (FCM) analysis of NIH/3T3 cells using KID Monoclonal antibody (blue) and negative control (red).
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Immunohistochemistry (IHC) analysis of paraffin-embedded rectum cancer tissues with DAB staining using KID Monoclonal antibody.
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ELISA analysis of KID antibody.
Protein Target Info & Infographic
Gene/Protein Information For KIF22 (Source: Uniprot.org, NCBI)
Gene Name
KIF22
Full Name
Kinesin-like protein KIF22
Weight
73262 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Kid; KIDA-328A3.2; kinesin family member 22; kinesin-like 4; Kinesin-like DNA-binding protein; Kinesin-like protein 4; kinesin-like protein KIF22; KNSL4kinesin-like DNA-binding protein pseudogene; OBP; OBP-1; OBP-2; origin of plasmid DNA replication-binding protein; oriP binding protein KIF22 A-328A3.2, KID, KNSL4, OBP, OBP-1, OBP-2, SEMDJL2 kinesin family member 22 kinesin-like protein KIF22|kinesin-like DNA-binding protein pseudogene|kinesin-like protein 4|oriP binding protein|origin of plasmid DNA replication-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIF22, check out the KIF22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIF22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KID KIF22 Monoclonal Antibody (A05570)
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No publications found for A05570
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-KID KIF22 Monoclonal Antibody
Question
We need to test anti-KID Monoclonal antibody A05570 on human brain lung for research purposes, then I may be interested in using anti-KID Monoclonal antibody A05570 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The products we sell, including anti-KID Monoclonal antibody A05570, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-12
Question
Does A05570 anti-KID Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
It shows on the product datasheet, A05570 anti-KID Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-KID Monoclonal antibody for IHC for human brain lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain lung identification?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
As indicated on the product datasheet, A05570 anti-KID Monoclonal antibody has been validated for Flow Cytometry, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-13
Question
We are currently using anti-KID Monoclonal antibody A05570 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The anti-KID Monoclonal antibody (A05570) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-15
Question
Is a blocking peptide available for product anti-KID Monoclonal antibody (A05570)?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
We do provide the blocking peptide for product anti-KID Monoclonal antibody (A05570). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-04
Question
Do you have a BSA free version of anti-KID Monoclonal antibody A05570 available?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-KID Monoclonal antibody A05570 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-03
Question
I see that the anti-KID Monoclonal antibody A05570 works with IHC, what is the protocol used to produce the result images on the product page?
J. Patel
Verified customer
Asked: 2014-05-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-05-12
