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Product Info Summary
| SKU: | M01717 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-KHDRBS1 Antibody (Center)
SKU/Catalog Number
M01717
Size
50 µl
Description
Boster Bio Anti-KHDRBS1 Antibody (Center) (Catalog # M01717). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KHDRBS1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01717)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This KHDRBS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 259-291 amino acids from the Central region of human KHDRBS1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01717 is reactive to KHDRBS1 in Human, Mouse
Reconstitution
Calculated molecular weight
48227 Da
Background of SAM68
Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed:22253824).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01717 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-KHDRBS1 Antibody (Center) at 1:2000 dilution
Lane 1: Raji whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Daudi whole cell lysate
Lane 4: HT-29 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 48 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M01717 staining KHDRBS1 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For KHDRBS1 (Source: Uniprot.org, NCBI)
Gene Name
KHDRBS1
Full Name
KH domain-containing, RNA-binding, signal transduction-associated protein 1
Weight
48227 Da
Superfamily
KHDRBS family
Alternative Names
KH domain-containing, RNA-binding, signal transduction-associated protein 1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, Sam68, p21 Ras GTPase-activating protein-associated p62, p68, KHDRBS1 (HGNC:18116) KHDRBS1 Sam68, p62, p68 KH RNA binding domain containing, signal transduction associated 1 KH domain-containing, RNA-binding, signal transduction-associated protein 1|GAP-associated tyrosine phosphoprotein p62 (Sam68)|KH domain containing, RNA binding, signal transduction associated 1|p21 Ras GTPase-activating protein-associated p62|src-associated in mitosis 68 kDa protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KHDRBS1, check out the KHDRBS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KHDRBS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KHDRBS1 Antibody (Center) (M01717)
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No publications found for M01717
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