Anti-Keratocan/KTN/Kera Antibody Picoband™

Product Name

Anti-Keratocan/KTN/Kera Antibody Picoband™

View all KERA Antibodies

SKU/Catalog Number

A07712-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Keratocan/KTN/Kera Antibody Picoband™ catalog # A07712-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Keratocan/KTN/Kera Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07712-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Keratocan/KTN/Kera recombinant protein (Position: R73-K115).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07712-1 is reactive to KERA in Mouse, Rat

Applications

A07712-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

41 kDa

Calculated molecular weight

49229 MW

Background of KERA

Keratocan (KTN) also known as keratan sulfate proteoglycan keratocan, is a protein that in humans is encoded by the KERA gene. The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Keratocan/KTN/Kera using anti-Keratocan/KTN/Kera antibody (A07712-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat eye tissue lysates,
Lane 2: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keratocan/KTN/Kera antigen affinity purified polyclonal antibody (Catalog # A07712-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keratocan/KTN/Kera at approximately 41 kDa. The expected band size for Keratocan/KTN/Kera is at 41 kDa.

Gene/Protein Information For KERA (Source: Uniprot.org, NCBI)

Alternative Names

CNA2; KERA; Keratocan; KTN; SLRR2B; SLRR2BKeratan sulfate proteoglycan keratocan KERA CNA2, KTN, SLRR2B keratocan keratocan|keratan sulfate proteoglycan keratocan

For more info on KERA, check out the KERA Infographic

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In this infographic, you will see the following information for KERA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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