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Product Info Summary
| SKU: | M00532-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody
SKU/Catalog Number
M00532-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody catalog # M00532-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00532-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DID-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KDM1 / LSD1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00532-1 is reactive to KDM1A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
92903 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00532-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
Positive Control
WB: Hela cell
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of KDM1/LSD1 expression in Hela cell lysate
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Immunohistochemical analysis of paraffin-embedded human colon, using KDM1/LSD1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For KDM1A (Source: Uniprot.org, NCBI)
Gene Name
KDM1A
Full Name
Lysine-specific histone demethylase 1A
Weight
92903 MW
Superfamily
flavin monoamine oxidase family
Alternative Names
Lysine-specific histone demethylase 1A;1.-.-.-;BRAF35-HDAC complex protein BHC110;Flavin-containing amine oxidase domain-containing protein 2;KDM1A;AOF2, KDM1, KIAA0601, LSD1; KDM1A AOF2, BHC110, CPRF, KDM1, LSD1 lysine demethylase 1A lysine-specific histone demethylase 1A|BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|[histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A|amine oxidase (flavin containing) domain 2|flavin-containing amine oxidase domain-containing protein 2|lysine (K)-specific demethylase 1A|lysine-specific histone demethylase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM1A, check out the KDM1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDM1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody (M00532-1)
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No publications found for M00532-1
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody
Question
My question regarding product M00532-1, anti-KDM1/LSD1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Edwards
Verified customer
Asked: 2018-12-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00532-1 anti-KDM1/LSD1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-28
Question
We are currently using anti-KDM1/LSD1 Rabbit Monoclonal antibody M00532-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
The anti-KDM1/LSD1 Rabbit Monoclonal antibody (M00532-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-KDM1/LSD1 Rabbit Monoclonal antibody M00532-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-01
