Anti-KDEL Receptor Monoclonal Antibody

Product Name

Anti-KDEL Receptor Monoclonal Antibody

View all KDELR1 Antibodies

SKU/Catalog Number

M07617-2

Size

100ug

Form

liquid

Description

Boster Bio Anti-KDEL Receptor Monoclonal Antibody catalog # M07617-2. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Chicken, Dog, Fruit fly, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Frog.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KDEL Receptor Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07617-2)

Host

Mouse

Contents

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

KR-10

Isotype

IgG1

Immunogen

A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~25kDa.

Reactive Species

M07617-2 is reactive to KDELR1 in Bovine, Chicken, Dog, Drosophila, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

60025 MW

Background of KDELR1

The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M07617-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For KDELR1 (Source: Uniprot.org, NCBI)

Alternative Names

ERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23 KDELR1 ERD2, ERD2.1, HDEL, PM23 KDEL endoplasmic reticulum protein retention receptor 1 ER lumen protein-retaining receptor 1|KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1|KDEL receptor 1|putative MAPK-activating protein PM23

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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