Click image to see more details
Product Info Summary
| SKU: | A05367-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-KCNT1 Antibody
SKU/Catalog Number
A05367-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-KCNT1 Antibody (Catalog# A05367-1). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCNT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05367-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human KCNT1. Immunogen sequence location: 1019-1068
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05367-1 is reactive to KCNT1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
138343 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05367-1 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KCNT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For KCNT1 (Source: Uniprot.org, NCBI)
Gene Name
KCNT1
Full Name
Potassium channel subfamily T member 1
Weight
138343 MW
Superfamily
potassium channel family
Alternative Names
KCNT1;KIAA1422;Potassium channel subfamily T member 1;KCa4.1 KCNT1 DEE14, EIEE14, ENFL5, KCa4.1, SLACK, Slo2.2, bA100C15.2 potassium sodium-activated channel subfamily T member 1 potassium channel subfamily T member 1|Sequence like a calcium-activated K+ channel|potassium channel, sodium activated subfamily T, member 1|potassium channel, subfamily T, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNT1, check out the KCNT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KCNT1 Antibody (A05367-1)
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No publications found for A05367-1
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Customer Q&As
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7 Customer Q&As for Anti-KCNT1 Antibody
Question
Does anti-KCNT1 antibody A05367-1 work on goat IHC with right hemisphere of cerebellum?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Our lab technicians have not tested anti-KCNT1 antibody A05367-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-KCNT1 antibody A05367-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat right hemisphere of cerebellum in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
We are currently using anti-KCNT1 antibody A05367-1 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The anti-KCNT1 antibody (A05367-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-24
Question
Do you have a BSA free version of anti-KCNT1 antibody A05367-1 available?
V. Rodriguez
Verified customer
Asked: 2019-10-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-KCNT1 antibody A05367-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-18
Question
I see that the anti-KCNT1 antibody A05367-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-30
Question
Would anti-KCNT1 antibody A05367-1 work for IHC with right hemisphere of cerebellum?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
According to the expression profile of right hemisphere of cerebellum, KCNT1 is highly expressed in right hemisphere of cerebellum. So, it is likely that anti-KCNT1 antibody A05367-1 will work for IHC with right hemisphere of cerebellum.
Boster Scientific Support
Answered: 2018-09-04
Question
Can you help my question with product A05367-1, anti-KCNT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05367-1 anti-KCNT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-19
Question
I am interested in to test anti-KCNT1 antibody A05367-1 on human right hemisphere of cerebellum for research purposes, then I may be interested in using anti-KCNT1 antibody A05367-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Gonzalez
Verified customer
Asked: 2015-11-06
Answer
The products we sell, including anti-KCNT1 antibody A05367-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-11-06
