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Product Info Summary
| SKU: | A04705-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-KCNQ5 Antibody Picoband®
SKU/Catalog Number
A04705-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KCNQ5 Antibody Picoband® catalog # A04705-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCNQ5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04705-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KCNQ5 recombinant protein (Position: E617-K932).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04705-1 is reactive to KCNQ5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
102 kDa
Calculated molecular weight
102.179kDa
Background of Kv7.5
Potassium voltage-gated channel subfamily KQT member 5 is a protein that in humans is encoded by the KCNQ5 gene. This gene is a member of the KCNQ potassium channel gene family that is differentially expressed in subregions of the brain and in skeletal muscle. KCNQ5 is mapped to 6q13. The protein encoded by this gene yields currents that activate slowly with depolarization and can form heteromeric channels with the protein encoded by the KCNQ3 gene. Currents expressed from this protein have voltage dependences and inhibitor sensitivities in common with M-currents. They are also inhibited by M1 muscarinic receptor activation. KCNQ5 has been shown to interact with KvLQT3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04705-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human PC-3 whole cell, human HEK293 whole cell
IHC: mouse brain tissue, rat brain tissue, human renal clear cell carcinoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KCNQ5 using anti-KCNQ5 antibody (A04705-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HEK293 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNQ5 antigen affinity purified polyclonal antibody (Catalog # A04705-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNQ5 at approximately 102KD. The expected band size for KCNQ5 is at 102KD.
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Figure 2. IHC analysis of KCNQ5 using anti-KCNQ5 antibody (A04705-1).
KCNQ5 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KCNQ5 Antibody (A04705-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of KCNQ5 using anti-KCNQ5 antibody (A04705-1).
KCNQ5 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KCNQ5 Antibody (A04705-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of KCNQ5 using anti-KCNQ5 antibody (A04705-1).
KCNQ5 was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KCNQ5 Antibody (A04705-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KCNQ5 (Source: Uniprot.org, NCBI)
Gene Name
KCNQ5
Full Name
Potassium voltage-gated channel subfamily KQT member 5
Weight
102.179kDa
Superfamily
potassium channel family
Alternative Names
Heat shock factor protein 2; HSF 2; Heat shock transcription factor 2; HSTF 2; HSF2; HSTF2 KCNQ5 Kv7.5, MRD46 potassium voltage-gated channel subfamily Q member 5 potassium voltage-gated channel subfamily KQT member 5|KQT-like 5|potassium channel protein|potassium channel subunit alpha KvLQT5|potassium channel, voltage gated KQT-like subfamily Q, member 5|voltage-gated potassium channel subunit Kv7.5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNQ5, check out the KCNQ5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNQ5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KCNQ5 Antibody Picoband® (A04705-1)
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