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Product Info Summary
| SKU: | PB9650 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KCNA3 Antibody Picoband®
SKU/Catalog Number
PB9650
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KCNA3 Antibody Picoband® catalog # PB9650. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCNA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9650)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human KCNA3, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9650 is reactive to KCNA3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
63842 MW
Background of Kv1.3
Potassium voltage-gated channel, shaker-related subfamily, member 3, also known as KCNA3 or Kv1.3, is a protein that in humans is encoded by the KCNA3 gene. This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T-cell proliferation and activation. This gene appears to be intronless and it is clustered together with KCNA2 and KCNA10 genes on chromosome 1. And Kv1.3 has been reported to be expressed in the inner mitochondrial membrane in lymphocytes. The apoptotic protein Bax has been suggested to insert into theouter mitochondrial membrane and occlude the pore of Kv1.3 via a lysine residue. Thus, Kv1.3 modulation may be one of many mechanisms that contribute to apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9650 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, K562 Whole Cell, HELA Whole Cell, 22RV1 Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KCNA3 using anti-KCNA3 antibody (PB9650).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: K562 Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug,
Lane 5: 22RV1 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA3 antigen affinity purified polyclonal antibody (Catalog # PB9650) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA3 at approximately 55 kDa. The expected band size for KCNA3 is at 64 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KCNA3 (Source: Uniprot.org, NCBI)
Gene Name
KCNA3
Full Name
Potassium voltage-gated channel subfamily A member 3
Weight
63842 MW
Superfamily
potassium channel family
Alternative Names
Potassium voltage-gated channel subfamily A member 3;HGK5;HLK3;HPCN3;Voltage-gated K (+) channel HuKIII;Voltage-gated potassium channel subunit Kv1.3;KCNA3;HGK5; KCNA3 HGK5, HLK3, HPCN3, HUKIII, KV1.3, MK3, PCN3 potassium voltage-gated channel subfamily A member 3 potassium voltage-gated channel subfamily A member 3|potassium channel 3|potassium channel, voltage gated shaker related subfamily A, member 3|potassium voltage-gated channel, shaker-related subfamily, member 3|type n potassium channel|voltage-gated K(+) channel HuKIII|voltage-gated potassium channel protein Kv1.3|voltage-gated potassium channel subunit Kv1.3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNA3, check out the KCNA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KCNA3 Antibody Picoband® (PB9650)
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1 Customer Q&As for Anti-KCNA3 Antibody Picoband®
Question
We are currently using anti-KCNA3 antibody PB9650 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
The anti-KCNA3 antibody (PB9650) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-30
