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- Table of Contents
Facts about Potassium voltage-gated channel subfamily A member 3.
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Human | |
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Gene Name: | KCNA3 |
Uniprot: | P22001 |
Entrez: | 3738 |
Belongs to: |
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potassium channel family |
HGK5; HLK3KV1.3; HPCN3PCN3; HUKIII; Kv1.3; MK3; potassium channel 3; potassium voltage-gated channel subfamily A member 3; potassium voltage-gated channel, shaker-related subfamily, member 3; type n potassium channel; Voltage-gated K(+) channel HuKIII; voltage-gated potassium channel protein Kv1.3; Voltage-gated potassium channel subunit Kv1.3
Mass (kDA):
63.842 kDA
Human | |
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Location: | 1p13.3 |
Sequence: | 1; NC_000001.11 (110653560..110674940, complement) |
Cell membrane; Multi-pass membrane protein.
Boster Bio primary antibodies have many benefits. Boster Bio's primary antibodies are validated to ensure specificity and affinity. They are also tested against known positive as well as negative samples. They offer generous product credits to scientists who first review their antibodies. This is an ideal opportunity to spread your positive or negative results among your peers. This way, you can reward scientists who discover new products before their competitors.
Boster Bio offers the highest-affinity primary antibodies against KC NA3 marker. The high-affinity antibodies are monoclonal or polyclonal, and have been used for over 25 years. These antibodies react with cells or particles and are available in a wide range of species and applications.
While there are a number of ways to generate antibodies, one of the most efficient ways to produce them is to immunize experimental animals with antigens and then fuse antibody-producing cells to generate a large amount of high-affinity antibodies. These antibodies can be synthesized by screening naive, synthetic, or phage libraries for their desired specificities.
Boster Bio provides high affinity primary antibodies for immunofluorescence. These antibodies have been validated for IHC, WB, ELISA, and FC. Boster also offers rabbit polyclonal antibodies as well as free secondary antibodies with any primary antibodies purchased. These antibodies retain their biological activity and are well-suited for a wide range of applications, including clinical and research.
Primary antibodies are immunoglobulins that specifically bind to an antigen of interest. Their specificity and affinity are usually used to determine their quality. Specificity refers the extent to which they bind the targeted antigen while affinity refers to how many unintended and unknown antigens they recognize. Good primary antibodies can measure, purify and detect the antigen in question.
While there are many reasons for using an antibody in immunofluorescence (IF), high-affinity antibodies work best for a variety applications. They are durable and can withstand heat up to 60°C. Primary antibodies can detect a specific antigen (bacterium or fungal). Because they're long-lasting, antibodies are used in immunohistochemistry. Monoclonal mouse antibodies have affinity constants between 10-6 and 10.
Imaging specific proteins is one of the most popular uses of primary antibodies in immunofluorescence. Boster Bio has high-affinity antibody to identify proteins in samples. Some are more specific than others and some even target phosphospecific proteins. These antibodies are highly effective for immunofluorescence experiments, which require a high level of specificity. They are especially useful when used in immunofluorescence research.
The type of protein and the antibody will influence the optimal coating conditions. Competition ELISA plates are coated using a higher concentration than the maximum binding capacity. This allows for a wider detection range. Other proteins, like rhoptry and fusion proteins should be coated at a lower amount to avoid nonspecific binding. Hooking can occur when these conditions are exceeded. Hooking is a phenomenon where proteins become stuck between the coating and the antigen, preventing efficient washing of the unbound protein.
To create a high quality antibody for Immunofluorescence experiments using immunofluorescence, you must first create a full-length rabbit antibodies. The antibody is then expressed in ExpiCHO cells. The antibody is then extracted using protein A column Chromatography. The purified antibody is then buffer exchanged into PBS to determine its concentration. Lowry's method is used to determine the affinity for the target progesterone in purified IgG. Once the clones demonstrate high affinity, further characterization can be performed.
High-affinity primary antibody are essential for a successful Immunofluorescence experiment. Different immunostaining procedures are used for validation of the antibodies. Some antibodies are very specific and can be used to detect multiple protein isoforms in cell cultures. Some antibodies are less specific and may produce a lower-affinity band. It is important to develop a specific antibody with carefully biochemical properties in order to ensure high-affinity antibodies for Immunofluorescence.
Boster Bio is a supplier high-affinity prima antibodies for ICC and ELISA as well IHC and other diagnostic procedures. Boster Bio also manufactures reagents such as buffers and lysates as well as assay kits. The company's reagents, kits and other products are manufactured in its own facility. This ensures that quality is maintained. Boster also uses proprietary trade secrets to increase the efficiency and sensitivity its products.
For ICC, it is critical to choose a good antibody with low cross-reactivity to the target protein. Boster Bio has a wide range of high-affinity, highly specific primary antibodies that can be used for this type of analysis. The company also offers custom-services and BeNeLux orders. Boster Bio is available to answer any questions you may have about purchasing a primary antigen.
The most important part in any ICC/IHC experiment, is the selection and use of a prima antibody. The proper visualization and analysis is possible only if the antibody selected is specific to the epitope being studied. Initial studies determine the best conditions for primary antibodies. The working dilution rate for polyclonal antibodies is lower than for monoclonal. To ensure minimal cross-reactivity, it is important to use working dilutions.
There are many commercial sources of primary antibodies. The quality of these antibodies can be variable and can impact the success of an experiment. To ensure that you get the best quality antibodies, it is important to independently verify the authenticity of the antibodies purchased. You can also obtain assistance from a commercial source such as R&D Systems. You should only buy high-affinity antibody directly from the manufacturer. You'll be able to control the commercial source.
You must ensure that the antibody chosen for ICC is very specific to the antigen. You should consider validation data, peer reviews, and end-user feedback when selecting the optimal antibodies. Choosing an antibody with low affinity can compromise staining and results in false positives. False positive results can result from an antibody that is cross-reactive to a target antigen.
PMID: 7829094 by Folander K., et al. Confirmation of the assignment of the gene encoding Kv1.3, a voltage- gated potassium channel (KCNA3) to the proximal short arm of human chromosome 1.
PMID: 1986382 by Philipson L.H., et al. Sequence and functional expression in Xenopus oocytes of a human insulinoma and islet potassium channel.