Anti-KAT3B/p300/EP300 Antibody Picoband®

KAT3B/p300 antibody

Boster Bio Anti-KAT3B/p300/EP300 Antibody Picoband® catalog # A00117. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00117
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-KAT3B/p300/EP300 Antibody Picoband®

View all KAT3B/p300 Antibodies

SKU/Catalog Number

A00117

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KAT3B/p300/EP300 Antibody Picoband® catalog # A00117. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-KAT3B/p300/EP300 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00117)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human KAT3B/p300/EP300, which shares 76.5% amino acid (aa) sequence identity with mouse EP300.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00117 is reactive to EP300 in Human, Mouse

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

264-300 kDa

Calculated molecular weight

21298 MW

Background of KAT3B/p300

E1A binding protein p300 also known as EP300 or p300 is a protein that in humans is encoded by the EP300 gene. The EP300 gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The EP300 protein appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. EP300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00117 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

Positive Control

WB: human 293T whole cell, mouse testis tissue
IHC: differentiated adenocarcinoma of the human rectum tissue, human classic Hodgkin's lymphoma tissue, human gastric signet ring cell carcinoma tissue, human placenta tissue
ICC/IF: SiHa cell
FCM: 293T cell

Validation Images & Assay Conditions

Gene/Protein Information For EP300 (Source: Uniprot.org, NCBI)

Gene Name

EP300

Full Name

Histone acetyltransferase p300

Weight

21298 MW

Alternative Names

GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;GTPase HRas, N-terminally processed;HRAS;HRAS1; EP300 KAT3B, MKHK2, RSTS2, p300 E1A binding protein p300 histone acetyltransferase p300|E1A-associated protein p300|E1A-binding protein, 300kD|histone butyryltransferase p300|histone crotonyltransferase p300|p300 HAT|protein 2-hydroxyisobutyryltransferase p300|protein propionyltransferase p300

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EP300, check out the EP300 Infographic

EP300 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EP300: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00117

100μg

$370
A00117-10ug

10μg sample (liquid)

$99
A00117-Biotin

100 μg Biotin conjugated

$570
A00117-Cy3

100 μg Cy3 conjugated

$570
A00117-Dylight488

100 μg Dylight488 conjugated

$570
A00117-Dylight550

100 μg Dylight550 conjugated

$570
A00117-Dylight594

100 μg Dylight594 conjugated

$570
A00117-FITC

100 μg FITC conjugated

$570
A00117-HRP

100 μg HRP conjugated

$570
A00117-APC

100 μg APC conjugated

$670
A00117-PE

100 μg PE conjugated

$670
A00117-iFluor647

100 μg iFluor647 conjugated

$670
A00117-carrier-free

Carrier Free

$370
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A00117
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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