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Product Info Summary
| SKU: | M00856 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-KAT13A / SRC1 Monoclonal Antibody
SKU/Catalog Number
M00856
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KAT13A / SRC1 Monoclonal Antibody catalog # M00856. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAT13A / SRC1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00856)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEHD-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KAT13A / SRC1 Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00856 is reactive to NCOA1 in Human
Applications
M00856 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
57513 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of KAT13A / SRC1 expression in HEK293 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For NCOA1 (Source: Uniprot.org, NCBI)
Gene Name
NCOA1
Full Name
Nuclear receptor coactivator 1
Weight
57513 MW
Superfamily
SRC/p160 nuclear receptor coactivator family
Alternative Names
bHLHe42; BHLHE74; Class E basic helix-loop-helix protein 74; EC 2.3.1.48; Hin-2 protein; KAT13A; MGC129719; NCoA-1; nuclear receptor coactivator 1; PAX3/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160bHLHe74F-SRC-1; SRC-1; SRC1MGC129720; Steroid receptor coactivator 1; steroid receptor coactivator-1 NCOA1 F-SRC-1, KAT13A, RIP160, SRC1, bHLHe42, bHLHe74 nuclear receptor coactivator 1 nuclear receptor coactivator 1|Hin-2 protein|class E basic helix-loop-helix protein 74|renal carcinoma antigen NY-REN-52|steroid receptor coactivator-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCOA1, check out the NCOA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCOA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KAT13A / SRC1 Monoclonal Antibody (M00856)
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No publications found for M00856
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-KAT13A / SRC1 Monoclonal Antibody
Question
Would anti-KAT13A / SRC1 Monoclonal antibody M00856 work for IP with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
According to the expression profile of cervix carcinoma erythroleukemia, NCOA1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-KAT13A / SRC1 Monoclonal antibody M00856 will work for IP with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-11-27
Question
I see that the anti-KAT13A / SRC1 Monoclonal antibody M00856 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-14
Question
Will M00856 anti-KAT13A / SRC1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
As indicated on the product datasheet, M00856 anti-KAT13A / SRC1 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-17
Question
I have a question about product M00856, anti-KAT13A / SRC1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00856 anti-KAT13A / SRC1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-01
Question
Is there a BSA free version of anti-KAT13A / SRC1 Monoclonal antibody M00856 available?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KAT13A / SRC1 Monoclonal antibody M00856 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-KAT13A / SRC1 Monoclonal antibody M00856. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-20
