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Product Info Summary
| SKU: | PA1709 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Kallikrein 1/KLK1 Antibody Picoband®
View all Kallikrein 1 Antibodies
SKU/Catalog Number
PA1709
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 1/KLK1 Antibody catalog # PA1709. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 1/KLK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1709)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Kallikrein 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1709 is reactive to Klk1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
28775 MW
Background of Kallikrein 1
KLK1 (KALLIKREIN 1), also called KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. KLK1 is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The KLK1 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and its exact cytogenetic location is 19q13.33. The KLK1 gene contains 5 coding exons. And KLK1 is the most centromeric gene in the cluster. Mice lacking tissue kallikrein are unable to generate significant levels of kinins in most tissues and develop cardiovascular abnormalities early in adulthood despite normal blood pressure. The protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1709 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat pancreas tissue, mouse pancreas tissue
IHC: Mouse Kidney Tissue
Validation Images & Assay Conditions
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Anti-Kallikrein 1 antibody, PA1709, Western blotting
Lane 1: Recombinant Mouse KLK1 Protein 10ng
Lane 2: Recombinant Mouse KLK1 Protein 5ng
Lane 3: Recombinant Mouse KLK1 Protein 2.5ng
Lane 4: Recombinant Mouse KLK1 Protein 1.25ng
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Anti-Kallikrein 1 antibody, PA1709, IHC(P)
IHC(P): Mouse Kidney Tissue
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Figure 3. Western blot analysis of KLK1 using anti-KLK1 antibody (PA1709).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: mouse pancreas tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLK1 antigen affinity purified polyclonal antibody (Catalog # PA1709) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLK1 at approximately 29KD. The expected band size for KLK1 is at 29KD.
Protein Target Info & Infographic
Gene/Protein Information For Klk1 (Source: Uniprot.org, NCBI)
Gene Name
Klk1
Full Name
Kallikrein-1
Weight
28775 MW
Superfamily
peptidase S1 family
Alternative Names
Kallikrein-1;3.4.21.35;Glandular kallikrein K1;KAL-B;Renal kallikrein;Tissue kallikrein-6;mGK-6;Klk1;Klk-6, Klk6; KLK1 KLKR, Klk6, hK1 kallikrein 1 kallikrein-1|glandular kallikrein 1|kallikrein 1, renal/pancreas/salivary|kallikrein serine protease 1|kidney/pancreas/salivary gland kallikrein|tissue kallikrein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Klk1, check out the Klk1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Klk1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 1/KLK1 Antibody Picoband® (PA1709)
Hello CJ!
PA1709 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dynamic involvement of ATG5 in cellular stress responses
Tracing tumorigenesis in a solid tumor model at single-cell resolution
Species: Mouse
The Goblet Cell Protein Clca1 (Alias mClca3 or Gob-5) Is Not Required for Intestinal Mucus Synthesis, Structure and Barrier Function in Naive or DSS-Challenged Mice
Dynamic involvement of ATG5 in cellular stress responses
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