Product Info Summary
SKU: | A12085 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC |
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Product info
Product Name
Anti-K0100 KIAA0100 Antibody
SKU/Catalog Number
A12085
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-K0100 KIAA0100 Antibody (Catalog # A12085). Tested in IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-K0100 KIAA0100 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12085)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human K0100.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12085 is reactive to KIAA0100 in Human
Reconstitution
Calculated molecular weight
253700 MW
Background of K0100
Belongs to the UPF0378 family. 4 isoforms of the human protein are produced by alternative splicing.?
Song J., Biochim. Biophys. Acta 1760:62-69(2006).
Nagase T., DNA Res. 2:37-43(1995).
Chen G., Leuk. Res. 29:503-509(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12085 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using K0100 antibody A12085.
Click image to see more details
Immunofluorescence analysis of MCF-7 cells, using K0100 antibody A12085.
Protein Target Info & Infographic
Gene/Protein Information For KIAA0100 (Source: Uniprot.org, NCBI)
Gene Name
KIAA0100
Full Name
Protein KIAA0100
Weight
253700 MW
Superfamily
SABRE family
Alternative Names
Protein KIAA0100;Antigen MLAA-22;Breast cancer-overexpressed gene 1 protein;KIAA0100;BCOX1; KIAA0100 BCOX, BCOX1, CT101, FMP27 KIAA0100 protein KIAA0100|U937-associated | MLAA-22|breast cancer overexpressed gene 1|breast cancer-overexpressed gene 1 protein|cancer/testis 101
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIAA0100, check out the KIAA0100 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIAA0100: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-K0100 KIAA0100 Antibody (A12085)
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No publications found for A12085
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Customer Q&As
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4 Customer Q&As for Anti-K0100 KIAA0100 Antibody
Question
I see that the anti-K0100 antibody A12085 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-31
Question
Will anti-K0100 antibody A12085 work on bovine IHC with mammary cancer?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Our lab technicians have not validated anti-K0100 antibody A12085 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-K0100 antibody A12085 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine mammary cancer in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-29
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-K0100 antibody A12085. Let me know if you need anything else.
M. Taylor
Verified customer
Asked: 2019-05-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
We are currently using anti-K0100 antibody A12085 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
The anti-K0100 antibody (A12085) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-24