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Product Info Summary
| SKU: | A11750 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-JM4 PRAF2 Antibody
SKU/Catalog Number
A11750
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-JM4 PRAF2 Antibody (Catalog # A11750). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JM4 PRAF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11750)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human JM4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11750 is reactive to PRAF2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
19258 MW
Background of PRAF2
May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.
Koichi Kusuhara, J. Virol., Oct 1999; 73: 8112 - 8119.
Isis Rivera-Walsh, J. Biol. Chem., Aug 2000; 275: 25222.
Eric D. Tang, J. Biol. Chem., Sep 2003; 278: 37297 - 37305.
Isis Rivera-Walsh, J. Biol. Chem., Oct 2001; 276: 40385 - 40388.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11750 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from A549 cells (Lane 2), using JM4 antiobdy A11750. The lane on the left is treated with systhesized peptide.
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Western blot analysis of extracts from LOVO cells, using JM4 antibody A11750.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using JM4 antibody A11750.
Protein Target Info & Infographic
Gene/Protein Information For PRAF2 (Source: Uniprot.org, NCBI)
Gene Name
PRAF2
Full Name
PRA1 family protein 2
Weight
19258 MW
Superfamily
PRA1 family
Alternative Names
PRA1 family protein 2;PRAF2;JM4; PRAF2 JM4, Yip6a PRA1 domain family member 2 PRA1 family protein 2|Jena-Muenchen 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRAF2, check out the PRAF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRAF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JM4 PRAF2 Antibody (A11750)
Hello CJ!
A11750 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis
Xiong W,Wu L,Tang R,Zhang Q,Guo Q,Song S.Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis.Cancer Manag Res.2021;13:4359-4371https://doi. org/10.2147/CMAR.S302084
Species: Human,Mouse
A11750 usage in article: APP:WB, SAMPLE:A431 CELL AND SCL-1 CELL, DILUTION:1:3000
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-JM4 PRAF2 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-JM4 antibody A11750. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
I have a question about product A11750, anti-JM4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11750 anti-JM4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-07
Question
I see that the anti-JM4 antibody A11750 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-22
Question
We are currently using anti-JM4 antibody A11750 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The anti-JM4 antibody (A11750) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-15
Question
Would A11750 anti-JM4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
You can see on the product datasheet, A11750 anti-JM4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-02
