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Product Info Summary
| SKU: | A02850-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-JHDM3A/JMJD2A/KDM4A Antibody Picoband®
SKU/Catalog Number
A02850-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® catalog # A02850-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02850-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human JHDM3A/JMJD2A/KDM4A recombinant protein (Position: D379-E1064).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02850-1 is reactive to KDM4A in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
36043 MW
Background of KDM4A
Lysine-specific demethylase 4A is an enzyme that in humans is encoded by the KDM4A gene. This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02850-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human 293T whole cell, human HEL whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
IHC: human ovarian serous carcinoma tissue, human squamous cell lung carcinoma tissue, human aryngeal squamous cell carcinoma tissue, mouse colon tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JHDM3A/JMJD2A/KDM4A antigen affinity purified polyclonal antibody (Catalog # A02850-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JHDM3A/JMJD2A/KDM4A at approximately 150 kDa. The expected band size for JHDM3A/JMJD2A/KDM4A is at 121 kDa.
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Figure 2. IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1).
JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human ovarian serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1).
JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1).
JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human aryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1).
JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. Flow Cytometry analysis of HepG2 cells using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1).
Overlay histogram showing HepG2 cells stained with A02850-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For KDM4A (Source: Uniprot.org, NCBI)
Gene Name
KDM4A
Full Name
Lysine-specific demethylase 4A
Weight
36043 MW
Superfamily
JHDM3 histone demethylase family
Alternative Names
gasdermin D, gasdermin-D KDM4A JHDM3A, JMJD2, JMJD2A, TDRD14A lysine demethylase 4A lysine-specific demethylase 4A|[histone H3]-trimethyl-L-lysine(36) demethylase 4A|[histone H3]-trimethyl-L-lysine(9) demethylase 4A|jmjC domain-containing histone demethylation protein 3A|jumonji C domain-containing histone demethylase 3A|jumonji domain containing 2|jumonji domain containing 2A|jumonji domain-containing protein 2A|lysine (K)-specific demethylase 4A|tudor domain containing 14A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM4A, check out the KDM4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDM4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® (A02850-1)
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