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Product Info Summary
| SKU: | A02068 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-JAM A pY280 F11R Antibody
SKU/Catalog Number
A02068
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-JAM A pY280 F11R Antibody (Catalog # A02068). Tested in ELISA, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. ?Aliquot contents and freeze at -20°C or below for extended storage. ?Avoid cycles of freezing and thawing. ?Centrifuge product if not completely clear after standing at room temperature. ?This product is stable for several weeks at 4°C as an undiluted liquid. ?Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-JAM A pY280 F11R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02068)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02068 is reactive to F11R in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
98809 MW
Background of JAM-A
Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02068 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 5 ug/ml
IF Microscopy: User optimized
WB: 1 ug/ml
Validation Images & Assay Conditions
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Immunofluorescence Microscopy of Rabbit anti-JAMA pY280 antibody.Tissue: T84 cells (untreated/treated).Fixation: 0.5% PFA. Antigen retrieval: not required.Primary antibody: JAMA pY280 antibody at 2
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Figure 1. Western blot analysis of F11R using anti-F11R antibody (A02068).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F11R antigen affinity purified polyclonal antibody (Catalog # A02068) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for F11R.
Protein Target Info & Infographic
Gene/Protein Information For F11R (Source: Uniprot.org, NCBI)
Gene Name
F11R
Full Name
Junctional adhesion molecule A
Weight
98809 MW
Superfamily
immunoglobulin superfamily
Alternative Names
JAM A pY280, JAM-A, Junctional adhesion molecule A, JAM-1, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, JAM1, JCAM, JAM 1, JAMA F11r|9130004G24, AA638916, ESTM3, ESTM33, JAM, JAM-, JAM-1, JAM-A, Jcam, Jcam1, Ly10, Ly106|F11 receptor|junctional adhesion molecule A|BV11 |F11 receptor/F11R|junction cell adhesion molecule A|junction cell adhesion molecule1|junctional adhesion molecule 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F11R, check out the F11R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F11R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JAM A pY280 F11R Antibody (A02068)
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