Click image to see more details
Product Info Summary
| SKU: | A00027-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Jak2 (Ab-570) Antibody
SKU/Catalog Number
A00027-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Jak2 (Ab-570) Antibody (Catalog # A00027-2). Tested in IHC applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Jak2 (Ab-570) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00027-2)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.568~572 (G-D-Y-G-Q) derived from Human JAK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00027-2 is reactive to JAK2 in Human, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
130674 MW
Background of JAK2
Plays a role in leptin signaling and control of body weight.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00027-2 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 125kd
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Figure 1. IHC analysis of JAK2 using anti-JAK2 antibody (A00027-2).
JAK2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-JAK2 Antibody (A00027-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For JAK2 (Source: Uniprot.org, NCBI)
Gene Name
JAK2
Full Name
Tyrosine-protein kinase JAK2
Weight
130674 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase JAK2;2.7.10.2;Janus kinase 2;JAK-2;JAK2; JAK2 JTK10 Janus kinase 2 tyrosine-protein kinase JAK2|JAK-2|Janus kinase 2 (a protein tyrosine kinase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JAK2, check out the JAK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Jak2 (Ab-570) Antibody (A00027-2)
Hello CJ!
A00027-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Song Y,Tang MY,Chen W,Wang Z,Wang SL.High JAK2 Protein Expression Predicts a Poor Prognosis in Patients with Resectable Pancreatic Ductal Adenocarcinoma.Dis Markers.2020 Sep 21;2020:7656031.doi:10. 1155/2020/7656031.PMID:33029256;PMCID:PMC7528024.
Species: Human
A00027-2 usage in article: APP:IHC, SAMPLE:PDAC TISSUE, DILUTION:1:100
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Jak2 (Ab-570) Antibody
Question
We have been able to see staining in rat erythroleukemia. Do you have any suggestions? Is anti-Jak2 (Ab-570) antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Based on literature erythroleukemia does express JAK2. Based on Uniprot.org, JAK2 is expressed in popliteal artery, fetal brain, erythroleukemia, among other tissues. Regarding which tissues have JAK2 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 10899310
Boster Scientific Support
Answered: 2020-01-03
Question
We are currently using anti-Jak2 (Ab-570) antibody A00027-2 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The anti-Jak2 (Ab-570) antibody (A00027-2) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-18
Question
Do you have a BSA free version of anti-Jak2 (Ab-570) antibody A00027-2 available?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Jak2 (Ab-570) antibody A00027-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-30
Question
See below the WB image, lot number and protocol we used for erythroleukemia using anti-Jak2 (Ab-570) antibody A00027-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-Jak2 (Ab-570) antibody (A00027-2)?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
We do provide the blocking peptide for product anti-Jak2 (Ab-570) antibody (A00027-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-21
Question
Our lab were happy with the WB result of your anti-Jak2 (Ab-570) antibody. However we have been able to see positive staining in popliteal artery endomembrane system using this antibody. Is that expected? Could you tell me where is JAK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Based on literature, popliteal artery does express JAK2. Generally JAK2 expresses in endomembrane system. Regarding which tissues have JAK2 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 10899310
Boster Scientific Support
Answered: 2019-08-06
Question
Would A00027-2 anti-Jak2 (Ab-570) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
It shows on the product datasheet, A00027-2 anti-Jak2 (Ab-570) antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-11
Question
I was wanting to use your anti-Jak2 (Ab-570) antibody for IHC for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
You can see on the product datasheet, A00027-2 anti-Jak2 (Ab-570) antibody has been validated for IHC on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-18
Question
My question regarding product A00027-2, anti-Jak2 (Ab-570) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00027-2 anti-Jak2 (Ab-570) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Jak2 (Ab-570) antibody A00027-2. Let me know if you need anything else.
B. Gonzalez
Verified customer
Asked: 2018-06-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-22
Question
Does anti-Jak2 (Ab-570) antibody A00027-2 work on horse IHC with fetal brain?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
Our lab technicians have not validated anti-Jak2 (Ab-570) antibody A00027-2 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Jak2 (Ab-570) antibody A00027-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse fetal brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-25
Question
I see that the anti-Jak2 (Ab-570) antibody A00027-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-24
Question
Would anti-Jak2 (Ab-570) antibody A00027-2 work for IHC with erythroleukemia?
J. Banerjee
Verified customer
Asked: 2017-01-23
Answer
According to the expression profile of erythroleukemia, JAK2 is highly expressed in erythroleukemia. So, it is likely that anti-Jak2 (Ab-570) antibody A00027-2 will work for IHC with erythroleukemia.
Boster Scientific Support
Answered: 2017-01-23
Question
Is this A00027-2 anti-Jak2 (Ab-570) antibody reactive to the isotypes of JAK2?
T. Patel
Verified customer
Asked: 2014-11-07
Answer
The immunogen of A00027-2 anti-Jak2 (Ab-570) antibody is Peptide sequence around aa.568~572 (G-D-Y-G-Q) derived from Human JAK2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-11-07
Question
I would like to test anti-Jak2 (Ab-570) antibody A00027-2 on human erythroleukemia for research purposes, then I may be interested in using anti-Jak2 (Ab-570) antibody A00027-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Miller
Verified customer
Asked: 2014-02-24
Answer
The products we sell, including anti-Jak2 (Ab-570) antibody A00027-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-24
