Click image to see more details
Product Info Summary
| SKU: | PB9647 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband®
SKU/Catalog Number
PB9647
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband® catalog # PB9647. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9647)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ITGA2B, different from the related mouse sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9647 is reactive to ITGA2B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120-135 kDa
Calculated molecular weight
113377 MW
Background of ITGA2B
Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. It is mapped to 17q21.32. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9647 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
WB: human HEL whole cell, human HEL whole cell, human K562 whole cell
IHC: mouse lung tissue, rat lung tissue, human mammary cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ITGA2B using anti-ITGA2B antibody (PB9647).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA2B antigen affinity purified polyclonal antibody (Catalog # PB9647) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA2B at approximately 120-135 kDa. The expected band size for ITGA2B is at 113 kDa.
Click image to see more details
Figure 2. IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647).
ITGA2B was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647).
ITGA2B was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647).
ITGA2B was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ITGA2B (Source: Uniprot.org, NCBI)
Gene Name
ITGA2B
Full Name
Integrin alpha-IIb
Weight
113377 MW
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha-IIb;GPalpha IIb;GPIIb;Platelet membrane glycoprotein IIb;CD41;Integrin alpha-IIb heavy chain;Integrin alpha-IIb light chain, form 1;Integrin alpha-IIb light chain, form 2;ITGA2B;GP2B, ITGAB; ITGA2B BDPLT16, BDPLT2, CD41, CD41B, GP2B, GPIIb, GT, GTA, HPA3, PPP1R93 integrin subunit alpha 2b integrin alpha-IIb|GPalpha IIb|alphaIIb protein|integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, CD41)|platelet fibrinogen receptor, alpha subunit|platelet glycoprotein IIb of IIb/IIIa complex|platelet membrane glycoprotein IIb|platelet-specific BAK|protein phosphatase 1, regulatory subunit 93
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ITGA2B, check out the ITGA2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGA2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband® (PB9647)
Hello CJ!
PB9647 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Thrombopoietic stimulating activity of rhTyrRS (Y341A)
Leila Revollo,Glenn Merrill-Skoloff,Karen De Ceunynck,James Dilks,Mattia Bordoli,Christian Peters,Leila Noetzli,Andreia Ionescu,Vicki Rosen,Joseph E. Italiano,Malcolm Whitman,Robert Flaumenhaft;The Secreted Tyrosine Kinase Vlk Is Essential for Normal Plat
Species: Human,Mouse
PB9647 usage in article: APP:WB, SAMPLE:293T CELL, DILUTION:NA
Thrombopoietic stimulating activity of rhTyrRS (Y341A)
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
2 Customer Q&As for Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband®
Question
What is the antigen retrieval method for PB9647 antibody in IHC-P applications?
Verified customer
Asked: 2020-09-09
Answer
The antigen retrieval method used for the Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband PB9647 is Heat Induced Epitope Retrieval (HIER). https://www.bosterbio.com/protocol-and-troubleshooting/ihc-protocol/ihc-p-protocol
Boster Scientific Support
Answered: 2020-09-09
Question
We are currently using anti-CD41/Integrin alpha 2b/ITGA2B antibody PB9647 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
The anti-CD41/Integrin alpha 2b/ITGA2B antibody (PB9647) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-11
