Product Info Summary
SKU: | A02969-4 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Islet 1/ISL1 Antibody Picoband™
SKU/Catalog Number
A02969-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Islet 1/ISL1 Antibody Picoband™ catalog # A02969-4. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Islet 1/ISL1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02969-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human ISL1 recombinant protein (Position: D118-Q289). Human Islet 1/ISL1 shares 100% amino acid (aa) sequence identity with both mouse and rat ISL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02969-4 is reactive to ISL1 in Human
Applications
A02969-4 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
Background of Islet-1
Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
![a02969 4 isl1 primary antibodies wb testing 1 a02969 4 isl1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a02969-4-isl1-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody (A02969-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Islet 1/ISL1 antigen affinity purified polyclonal antibody (Catalog # A02969-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Islet 1/ISL1 at approximately 50 kDa. The expected band size for Islet 1/ISL1 is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ISL1 (Source: Uniprot.org, NCBI)
Gene Name
ISL1
Full Name
Insulin gene enhancer protein ISL-1
Weight
Alternative Names
insulin gene enhancer protein ISL-1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain; ISL1 transcription factor, LIM/homeodomain, (islet-1); ISL1; Isl-1; Islet1; Islet-1 ISL1 ISLET1, Isl-1 ISL LIM homeobox 1 insulin gene enhancer protein ISL-1|islet-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ISL1, check out the ISL1 Infographic
![ISL1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ISL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Islet 1/ISL1 Antibody Picoband™ (A02969-4)
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