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Product Info Summary
| SKU: | A10787 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ISL2/Islet 2 Antibody
SKU/Catalog Number
A10787
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ISL2/Islet 2 Antibody (Catalog # A10787). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ISL2/Islet 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10787)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ISL2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10787 is reactive to ISL2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
39768 MW
Background of Islet-2
Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways By similarity.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10787 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using ISL2 antibody A10787.
Protein Target Info & Infographic
Gene/Protein Information For ISL2 (Source: Uniprot.org, NCBI)
Gene Name
ISL2
Full Name
Insulin gene enhancer protein ISL-2
Weight
39768 MW
Alternative Names
Insulin gene enhancer protein ISL-2;Islet-2;ISL2; Isl2|ISL LIM homeobox 2|insulin gene enhancer protein ISL-2|insulin related protein 2 (islet 2)|islet-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ISL2, check out the ISL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ISL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ISL2/Islet 2 Antibody (A10787)
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No publications found for A10787
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ISL2/Islet 2 Antibody
Question
Would anti-ISL2/Islet 2 antibody A10787 work on horse WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
Our lab technicians have not validated anti-ISL2/Islet 2 antibody A10787 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ISL2/Islet 2 antibody A10787 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-29
Question
Is there a BSA free version of anti-ISL2/Islet 2 antibody A10787 available?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ISL2/Islet 2 antibody A10787 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-05
Question
See attached the WB image, lot number and protocol we used for ectocervix using anti-ISL2/Islet 2 antibody A10787. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-29
Question
Is this A10787 anti-ISL2/Islet 2 antibody reactive to the isotypes of ISL2?
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
The immunogen of A10787 anti-ISL2/Islet 2 antibody is Synthesized peptide derived from internal of human ISL2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-24
Question
We are currently using anti-ISL2/Islet 2 antibody A10787 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
C. Thomas
Verified customer
Asked: 2018-03-27
Answer
The anti-ISL2/Islet 2 antibody (A10787) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-27
Question
I see that the anti-ISL2/Islet 2 antibody A10787 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-05
Question
Does anti-ISL2/Islet 2 antibody A10787 work for WB with ectocervix?
D. Roberts
Verified customer
Asked: 2013-02-19
Answer
According to the expression profile of ectocervix, ISL2 is highly expressed in ectocervix. So, it is likely that anti-ISL2/Islet 2 antibody A10787 will work for WB with ectocervix.
Boster Scientific Support
Answered: 2013-02-19
