Product Info Summary
SKU: | A12588-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IRX1 Antibody Picoband®
SKU/Catalog Number
A12588-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IRX1 Antibody Picoband® catalog # A12588-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IRX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12588-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Standard Protein
E.coli-derived human IRX1 recombinant protein (Position: Q95-A480). Human IRX1 shares 89.9% amino acid (aa) sequence identity with mouse IRX1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12588-2 is reactive to Irx1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
50 kDa
Background of Irx1
Iroquois-class homeodomain protein IRX-1, also known as Iroquois homeobox protein 1, is a protein that in humans is encoded by the IRX1 gene. This gene encodes a member of the Iroquois homeobox protein family. Homeobox genes in this family are involved in pattern formation in the embryo. The gene product has been identified as a tumor suppressor in gastric and head and neck cancers. A pseudogene of this gene is located on chromosome 13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12588-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human U251 whole cell, human Hacat whole cell, rat lung tissue, rat brain tissue, rat C6 whole cell, mouse lung tissue, mouse brain tissue, mouse LLC tissue
Validation Images & Assay Conditions
![a12588 2 irx1 primary antibodies wb testing 1 a12588 2 irx1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a12588-2-irx1-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of IRX1 using anti-IRX1 antibody (A12588-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse LLC tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRX1 antigen affinity purified polyclonal antibody (Catalog # A12588-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRX1 at approximately 50 kDa. The expected band size for IRX1 is at 50 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Irx1 (Source: Uniprot.org, NCBI)
Gene Name
Irx1
Full Name
Iroquois-class homeodomain protein IRX-1
Weight
Superfamily
TALE/IRO homeobox family
Alternative Names
Homeodomain protein IRXA1; iroquois homeobox 1; Iroquois homeobox protein 1; iroquois-class homeodomain protein IRX-1; IRX-5; IRXA1 Irx1|Iroquois homeobox 1|iroquois-class homeodomain protein IRX-1|Iroquois related homeobox 1|homeodomain protein IRXA1|iroquois homeobox protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Irx1, check out the Irx1 Infographic
![Irx1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Irx1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRX1 Antibody Picoband® (A12588-2)
Hello CJ!
No publications found for A12588-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IRX1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IRX1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question