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Product Info Summary
| SKU: | A00268 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-IRS-1 (Ab-636) Antibody
SKU/Catalog Number
A00268
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-IRS-1 (Ab-636) Antibody (Catalog # A00268). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRS-1 (Ab-636) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00268)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.634~638 (P-M-S-P-K) derived from Human IRS-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00268 is reactive to IRS1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
23954 MW
Background of IRS1
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76
Kadowaki T, et al. (2000) J Clin Invest; 106(4): 459-465
Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645
Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00268 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 180kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells using IRS-1(Ab-636) Antibody #A02268.
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Immunofluorescence staining of methanol-fixed Hela cells using IRS-1(Ab-636) Antibody #A00268.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1(Ab-636) Antibody #A02268(left) or the same antibody preincubated with blocking peptide(right).
Protein Target Info & Infographic
Gene/Protein Information For IRS1 (Source: Uniprot.org, NCBI)
Gene Name
IRS1
Full Name
Insulin receptor substrate 1
Weight
23954 MW
Alternative Names
Single-stranded DNA cytosine deaminase;3.5.4.38;Activation-induced cytidine deaminase;Cytidine aminohydrolase;AICDA;AID; IRS1 HIRS-1 insulin receptor substrate 1 insulin receptor substrate 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRS1, check out the IRS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRS-1 (Ab-636) Antibody (A00268)
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No publications found for A00268
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-IRS-1 (Ab-636) Antibody
Question
I see that the anti-IRS-1 (Ab-636) antibody A00268 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-05-01
Question
I have attached the WB image, lot number and protocol we used for eye using anti-IRS-1 (Ab-636) antibody A00268. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
Is this A00268 anti-IRS-1 (Ab-636) antibody reactive to the isotypes of IRS1?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The immunogen of A00268 anti-IRS-1 (Ab-636) antibody is Peptide sequence around aa.634~638 (P-M-S-P-K) derived from Human IRS-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-14
Question
I have a question about product A00268, anti-IRS-1 (Ab-636) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00268 anti-IRS-1 (Ab-636) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-11
Question
Does A00268 anti-IRS-1 (Ab-636) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
You can see on the product datasheet, A00268 anti-IRS-1 (Ab-636) antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-28
Question
Do you have a BSA free version of anti-IRS-1 (Ab-636) antibody A00268 available?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IRS-1 (Ab-636) antibody A00268 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-02
Question
We ordered your anti-IRS-1 (Ab-636) antibody for IHC on cervix carcinoma last year. I am using human, and I plan to use the antibody for WB next. you antibody examining cervix carcinoma as well as eye in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
I looked at the website and datasheets of our anti-IRS-1 (Ab-636) antibody and I see that A00268 has been validated on human in both IHC and WB. Thus A00268 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-16
Question
I am interested in using your anti-IRS-1 (Ab-636) antibody for insulin-like growth factor receptor signaling pathway studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
We appreciate your inquiry. This A00268 anti-IRS-1 (Ab-636) antibody is validated on hela cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-15
Question
We are currently using anti-IRS-1 (Ab-636) antibody A00268 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
The anti-IRS-1 (Ab-636) antibody (A00268) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-17
Question
I was wanting to use your anti-IRS-1 (Ab-636) antibody for IHC for rat eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat eye identification?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
You can see on the product datasheet, A00268 anti-IRS-1 (Ab-636) antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-IRS-1 (Ab-636) antibody A00268. Let me know if you need anything else.
G. Carter
Verified customer
Asked: 2018-01-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-24
Question
We have been able to see staining in rat cervix carcinoma. Do you have any suggestions? Is anti-IRS-1 (Ab-636) antibody supposed to stain cervix carcinoma positively?
D. Jones
Verified customer
Asked: 2016-08-09
Answer
Based on literature cervix carcinoma does express IRS1. Based on Uniprot.org, IRS1 is expressed in endometrium, skeletal muscle, eye, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have IRS1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 8513971
Boster Scientific Support
Answered: 2016-08-09
Question
Is a blocking peptide available for product anti-IRS-1 (Ab-636) antibody (A00268)?
T. Yang
Verified customer
Asked: 2016-03-29
Answer
We do provide the blocking peptide for product anti-IRS-1 (Ab-636) antibody (A00268). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-03-29
Question
Does anti-IRS-1 (Ab-636) antibody A00268 work for IHC with eye?
B. Brown
Verified customer
Asked: 2014-09-04
Answer
According to the expression profile of eye, IRS1 is highly expressed in eye. So, it is likely that anti-IRS-1 (Ab-636) antibody A00268 will work for IHC with eye.
Boster Scientific Support
Answered: 2014-09-04
Question
I would like to test anti-IRS-1 (Ab-636) antibody A00268 on rat eye for research purposes, then I may be interested in using anti-IRS-1 (Ab-636) antibody A00268 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Parker
Verified customer
Asked: 2014-08-13
Answer
The products we sell, including anti-IRS-1 (Ab-636) antibody A00268, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-13
