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Product Info Summary
| SKU: | PB9645 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IRF2 Antibody Picoband®
SKU/Catalog Number
PB9645
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IRF2 Antibody Picoband® catalog # PB9645. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9645)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IRF2, different from the related mouse sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9645 is reactive to IRF2 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
39354 MW
Background of IRF2
IRF2 (interferon regulatory factor 2) is a member of the interferon regulatory transcription factor (IRF) family. The IRF2 gene is mapped on 4q35.1. When the IRF2 gene was overexpressed in NIH 3T3 cells, the cells became transformed and displayed enhanced tumorigenicity in nude mice. One IRF binding site was found within the IRF2 promoter, and expression of the IRF2 gene was affected by both transient and stable IRF1 expression. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. Irf2 was required to prevent NK-cell apoptosis and keep immature NK cells alive, thus promoting NK-cell maturation and their supply to peripheral blood.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9645 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Intestine Tissue, SW620 Whole Cell, COLO320 Whole Cell, HELA Whole Cell, A549 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IRF2 using anti-IRF2 antibody (PB9645).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Intestine Tissue Lysate at 50ug,
Lane 2: SW620 Whole Cell Lysate at 40ug,
Lane 3: COLO320 Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug,
Lane 5: A549 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF2 antigen affinity purified polyclonal antibody (Catalog # PB9645) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF2 at approximately 50 kDa. The expected band size for IRF2 is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For IRF2 (Source: Uniprot.org, NCBI)
Gene Name
IRF2
Full Name
Interferon regulatory factor 2
Weight
39354 MW
Superfamily
IRF family
Alternative Names
Interferon regulatory factor 2;IRF-2;IRF2; IRF2 IRF-2 interferon regulatory factor 2 interferon regulatory factor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRF2, check out the IRF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRF2 Antibody Picoband® (PB9645)
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Customer Q&As
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1 Customer Q&As for Anti-IRF2 Antibody Picoband®
Question
We are currently using anti-IRF2 antibody PB9645 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The anti-IRF2 antibody (PB9645) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-06
